Characterization of the sdw1 semi-dwarf gene in barley

BMC Plant Biology

Research Article

George Yan¹  Sue Broughton²  Wenying Zhang³  Yanhao Xu⁴  Chengdao Li⁵  Qiaojun Jia⁶  Tefera Angessa⁷  Gaofeng Zhou⁷  Xiao-Qi Zhang⁷ 

[1] College of Horticultural and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China;Department of Agriculture and Food Government of Western Australia, WA6150, South Perth, Australia;Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, 434000, Jingzhou, Hubei, China;Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, 434000, Jingzhou, Hubei, China;Western Barley Genetics Alliance, Murdoch University, WA6150, Murdoch, Australia;Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, 434000, Jingzhou, Hubei, China;Western Barley Genetics Alliance, Murdoch University, WA6150, Murdoch, Australia;Department of Agriculture and Food Government of Western Australia, WA6150, South Perth, Australia;Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province /College of Life Sciences, Zhejiang Sci-Tech University, 310018, Hangzhou, China;Western Barley Genetics Alliance, Murdoch University, WA6150, Murdoch, Australia;

关键词: sdw1;    Functional gene;    Allelic variation;    Diagnostic marker;    Functional compensation;   

DOI  :  10.1186/s12870-016-0964-4

 received in 2016-01-26, accepted in 2016-12-23,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe dwarfing gene sdw1 has been widely used throughout the world to develop commercial barley varieties. There are at least four different alleles at the sdw1 locus.ResultsMutations in the gibberellin 20-oxidase gene (HvGA20ox2) resulted in multiple alleles at the sdw1 locus. The sdw1.d allele from Diamant is due to a 7-bp deletion in exon 1, while the sdw1.c allele from Abed Denso has 1-bp deletion and a 4-bp insertion in the 5’ untranslated region. The sdw1.a allele from Jotun resulted from a total deletion of the HvGA20ox2 gene. The structural changes result in lower gene expression in sdw1.d and lack of expression in sdw1.a. There are three HvGA20ox genes in the barley genome. The partial or total loss of function of the HvGA20ox2 gene could be compensated by enhanced expression of its homolog HvGA20ox1and HvGA20ox3. A diagnostic molecular marker was developed to differentiate between the wild-type, sdw1.d and sdw1.a alleles and another molecular marker for differentiation of sdw1.c and sdw1.a. The markers were further tested in 197 barley varieties, out of which 28 had the sdw1.d allele and two varieties the sdw1.a allele. To date, the sdw1.d and sdw1.a alleles have only been detected in the modern barley varieties and lines.ConclusionsThe results provided further proof that the gibberellin 20-oxidase gene (HvGA20ox2) is the functional gene of the barley sdw1 mutants. Different deletions resulted in different functional alleles for different breeding purposes. Truncated protein could maintain partial function. Partial or total loss of function of the HvGA20ox2 gene could be compensated by enhanced expression of its homolog HvGA20ox1 and HvGA20ox3.

【 授权许可】

CC BY   
© The Author(s). 2017