BMC Complementary and Alternative Medicine | |
Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae) | |
Research Article | |
Thaciane da Cunha Soares1  Silvana Maria Zucolotto1  Manoela Torres-Rêgo2  Mariana Angélica Oliveira Bitencourt2  Maira Conceição Jerônimo de Souza Lima2  Arnóbio Antônio da Silva-Júnior2  Rafael Caetano Lisbôa Castro de Andrade2  Matheus de Freitas Fernandes-Pedrosa2  Allanny Alves Furtado2  José Carlos Tomaz3  Norberto Peporine Lopes3  Eduardo Pereira de Azevedo4  | |
[1] Departamento de Farmácia, Laboratório de Farmacognosia, Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, Petrópolis, CEP 59012-570, Natal, RN, Brazil;Departamento de Farmácia, Laboratório de Tecnologia e Biotecnologia Farmacêutica (TecBioFar), Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Farias, s/n, Petrópolis, CEP 59012-570, Natal, RN, Brazil;Departamento de Física e Química, Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, Monte Alegre, CEP 14040-903, Ribeirão Preto, SP, Brazil;Departamento de Pós-graduação em Biotecnologia, Universidade Potiguar, Avenida Senador Salgado Filho, 1610, Lagoa Nova, CEP 59056-000, Natal, RN, Brazil; | |
关键词: Anti-inflammatory; Apocynaceae; Chlorogenic acid; Hancornia speciosa; Mangabeira; Rutin; | |
DOI : 10.1186/s12906-016-1259-x | |
received in 2016-02-15, accepted in 2016-08-03, 发布年份 2016 | |
来源: Springer | |
【 摘 要 】
BackgroundHancornia speciosa Gomes (Apocynaceae), popularly known as “mangabeira,” has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatment of ulcers and inflammatory disorders, only few studies have been conducted to prove such biological activities. This study investigated the anti-inflammatory properties of the aqueous extract of the fruits of H. speciosa Gomes as well as its bioactive compounds using in vivo experimental models.MethodsThe bioactive compounds were identified by High Performance Liquid Chromatography coupled with diode array detector (HPLC-DAD) and Liquid Chromatography coupled with Mass Spectrometry (LC-MS). The anti-inflammatory properties were investigated through in vivo tests, which comprised xylene-induced ear edema, carrageenan-induced peritonitis and zymosan-induced air pouch. The levels of IL-1β, IL-6, IL-12 and TNF-α were determined using ELISA.ResultsRutin and chlorogenic acid were identified in the extract as the main secondary metabolites. In addition, the extract as well as rutin and chlorogenic acid significantly inhibited the xilol-induced ear edema and also reduced the cell migration in both carrageenan-induced peritonitis and zymosan-induced air pouch models. Reduced levels of cytokines were also observed.ConclusionThis is the first study that demonstrated the anti-inflammatory activity of the extract of H. speciosa fruits against different inflammatory agents in animal models, suggesting that its bioactive molecules, especially rutin and chlorogenic acid are, at least in part, responsible for such activity. These findings support the widespread use of Hancornia speciosa in popular medicine and demonstrate that its aqueous extract has therapeutical potential for the development of herbal drugs with anti-inflammatory properties.
【 授权许可】
CC BY
© The Author(s). 2016
【 预 览 】
Files | Size | Format | View |
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