Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts

BMC Complementary and Alternative Medicine

Research Article

Francesca Pintus¹  Antonella Fais¹  Benedetta Era¹  Rosaria Medda¹  Amalia Di Petrillo¹  Celestino Santos-Buelga²  Ana Maria González-Paramás² 

[1] Department of Life and Environmental Sciences, University of Cagliari, SS 554, Bivio per Sestu, I-09042 Monserrato, Cagliari, Italy;Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain;

关键词: Antioxidants;    Asphodelus microcarpus;    B16F10 melanoma cells;    Flavonoids;    Polyphenols;    Tyrosinase inhibitors;   

DOI  :  10.1186/s12906-016-1442-0

 received in 2016-06-21, accepted in 2016-10-25,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAsphodelus microcarpus belongs to the family Liliaceae that include several medicinal plants. In the traditional medicine plants of the genus Asphodelus are used to treat skin disorders such as ectodermal parasites, psoriasis, microbial infection and for lightening freckles. In order to find novel skin depigmenting agents, the present work was carry out to evaluate antioxidant activity and tyrosinase inhibitory potential of leaves, flowers and tubers extracts of A. microcarpus. The phytochemical composition of the active extract was also evaluated.MethodsThree different extracts (water, methanol and ethanol) from leaves, flowers and tubers of A. microcarpus were evaluated for their inhibitory effect on tyrosinase activity using l-3,4-dihydroxyphenylalanine (l-DOPA) as substrate. Inhibition of cellular tyrosinase activity and melanin production was also investigated in melanoma B16F10 cells. Antioxidant activity, total phenolic and flavonoids contents were determined using standard in vitro methods. HPLC-DAD-MS was used to identify phenolic profile of the active extract.ResultsThe results showed that all extracts have a direct inhibitory anti-tyrosinase activity, with ethanolic extract from flowers (FEE) exhibiting the stronger effect. Kinetic analysis revealed that FEE acts as an uncompetitive inhibitor with a Ki value of 0.19 mg/mL. The same effect was observed in murine melanoma B16F10 cells. Cellular tyrosinase activity as well as melanin content were reduced in FEE-treated cells. The results were comparable to that of the standard tyrosinase inhibitor (kojic acid). Furthermore, the same extract showed the highest antioxidant activity and an elevated levels of total phenolics and flavonoid content. Eleven phenolic components were identified as chlorogenic acid, luteolin derivates, naringenin and apigenin.ConclusionsOur findings showed that FEE from A. microcarpus inhibits tyrosinase and exerted antimelanogenesis effect in B16F10 cells. This extract also showed the highest scavenging activity, which could be mainly attributed to its high levels of total polyphenols and flavonoids. These results suggest that A. microcarpus has a great potential as sources of bioactive compounds which could be used as depigmenting agents in skin disorders.

【 授权许可】

CC BY   
© The Author(s). 2016