(Not) Keeping the stem straight: a proteomic analysis of maritime pine seedlings undergoing phototropism and gravitropism

BMC Plant Biology

Research Article

Thierry Fourcaud¹  Alexia Stokes²  Celine Lalanne³  Franck Salin³  Christophe Plomion³  Catherine Krier³  ElHadji Maodo Ba⁴  Raul Herrera⁵  Stéphane Claverol⁶ 

[1] CIRAD, UMR AMAP, 34000, Montpellier, France;INRA, UMR AMAP, 34000, Montpellier, France;INRA, UMR Biogeco 1202, 69 route d'Arcachon, 33612, Cestas, France;Inspection Régionale des Eaux et Forêts de Kolda, 57, Bp, Kolda, Senegal;Instituto Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile;Pôle protéomique de la Plateforme Génomique Fonctionnelle Bordeaux, Université Bordeaux 2, Bordeaux, France;

关键词: Compression Wood;    Tension Wood;    Apical Shoot;    Gravitropic Response;    Stem Apex;   

DOI  :  10.1186/1471-2229-10-217

 received in 2010-01-19, accepted in 2010-10-06,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPlants are subjected to continuous stimuli from the environment and have evolved an ability to respond through various growth and development processes. Phototropism and gravitropism responses enable the plant to reorient with regard to light and gravity.ResultsWe quantified the speed of maritime pine seedlings to reorient with regard to light and gravity over 22 days. Seedlings were inclined at 15, 30 and 45 degrees with vertical plants as controls. A lateral light source illuminated the plants and stem movement over time was recorded. Depending on the initial angle of stem lean, the apical response to the lateral light source differed. In control and 15° inclined plants, the apex turned directly towards the light source after only 2 h. In plants inclined at 30° and 45°, the apex first reoriented in the vertical plane after 2 h, then turned towards the light source after 24 h. Two-dimensional gel electrophoresis coupled with mass spectrometry was then used to describe the molecular response of stem bending involved in photo- and gravi-tropism after 22 hr and 8 days of treatment. A total of 486 spots were quantitatively analyzed using image analysis software. Significant changes were determined in the protein accumulation of 68 protein spots. Early response gravitropic associated proteins were identified, which are known to function in energy related and primary metabolism. A group of thirty eight proteins were found to be involved in primary metabolism and energy related metabolic pathways. Degradation of Rubisco was implicated in some protein shifts.ConclusionsOur study demonstrates a rapid gravitropic response in apices of maritime pine seedlings inclined >30°. Little or no response was observed at the stem bases of the same plants. The primary gravitropic response is concomitant with a modification of the proteome, consisting of an over accumulation of energy and metabolism associated proteins, which may allow the stem to reorient rapidly after bending.

【 授权许可】

Unknown   
© Herrera et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.