Oxaliplatin long-circulating liposomes improved therapeutic index of colorectal carcinoma

BMC Biotechnology

Research Article

Zhong X Fu¹  Wei D Lu¹  Chuang Yang²  Hai Z Liu³ 

[1] Department of Gastrointestinal Surgery, First Affiliated Hospital, Chongqing Medical University, 400016, Chongqing, Chongqing, China;Department of Gastrointestinal Surgery, First Affiliated Hospital, Chongqing Medical University, 400016, Chongqing, Chongqing, China;Department of Hepatobiliary Surgery, Mianyang Third People's Hospital, 621000, Mianyang, Sichuan Province, China;Departments of Gynecology and Obstetrics, Second Affiliated Hospital, Chongqing Medical University, 400016, Chongqing, Chongqing, China;

关键词: Oxaliplatin;    SW480 Cell;    Entrapment Efficiency;    Human Colorectal Carcinoma Cell Line;    Target Tumour Tissue;   

DOI  :  10.1186/1472-6750-11-21

 received in 2010-09-28, accepted in 2011-03-15,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCytotoxic drugs are non-selective between normal and pathological tissue, and this poses a challenge regarding the strategy for treatment of tumors. To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. We investigated the ability of oxaliplatin long-circulating liposomes (PEG-liposomal L-oHP) to provide an improved therapeutic index of colorectal carcinoma.ResultsWe determined that PEG- liposomes conjugated with cells at 2 h, with a mean fluorescence intensity that was enhanced upon extended induction time. The PEG-liposomal L-oHP induced a significant apoptotic response as compared with free L-oHP, 23.21% ± 3.38% vs. 16.85% ± 0.98%, respectively. Fluorescence imaging with In-Vivo Imaging demonstrated that PEG- liposomes specifically targeted tumour tissue. After intravenous injections of PEG-liposomal L-oHP or free L-oHP, the tumour volume suppression ratio was 26.08% ± 12.43% and 18.19% ± 7.09%, respectively, the percentage increased life span (ILS%) was 45.36% and 76.19%, respectively, and Bcl-2, Bax mRNA and protein expression in tumour tissue was 0.27-fold vs. 0.88-fold and 1.32-fold vs. 1.61-fold compared with free L-oHP, respectively.ConclusionThe PEG-liposomal L-oHP exhibited a tendency to target tumour tissue and demonstrated a significantly greater impact on apoptosis compared to free oxaliplatin.

【 授权许可】

Unknown   
© Yang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.