Transcriptional regulatory networks controlling woolliness in peach in response to preharvest gibberellin application and cold storage

BMC Plant Biology

Research Article

Cesar Valmor Rombaldi¹  Fábio C. Chaves¹  Livio Trainotti²  Alice Tadiello³  Vera Quecini⁴  César L. Girardi⁴  Antonio Costa de Oliveira⁵  Camila Pegoraro⁶ 

[1] Departament of Food Science and Technology, Universidade Federal de Pelotas, Campus UFPel Capão do Leão, 96010-900, Pelotas, RS, Brazil;Department of Biology, University of Padova, Viale G. Colombo, 3, 35121, Padova, Italy;Department of Biology, University of Padova, Viale G. Colombo, 3, 35121, Padova, Italy;Current Address: Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, San Michele all’Adige, 38010, Trento, Italy;Embrapa Uva e Vinho, Rua Livramento 515, 95700-000, Bento Gonçalves, RS, Brazil;Plant Genomics and Breeding Center, Universidade Federal de Pelotas, Campus UFPel Capão do Leão, 96010-900, Pelotas, RS, Brazil;Plant Genomics and Breeding Center, Universidade Federal de Pelotas, Campus UFPel Capão do Leão, 96010-900, Pelotas, RS, Brazil;Current Address: Embrapa Uva e Vinho, Rua Livramento 515, 95700-000, Bento Gonçalves, RS, Brazil;

关键词: Chilling injury;    Cold storage;    Genome wide gene expression;    Gibberellic acid;    Prunus persica;   

DOI  :  10.1186/s12870-015-0659-2

 received in 2015-07-31, accepted in 2015-11-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPostharvest fruit conservation relies on low temperatures and manipulations of hormone metabolism to maintain sensory properties. Peaches are susceptible to chilling injuries, such as ‘woolliness’ that is caused by juice loss leading to a ‘wooly’ fruit texture. Application of gibberellic acid at the initial stages of pit hardening impairs woolliness incidence, however the mechanisms controlling the response remain unknown. We have employed genome wide transcriptional profiling to investigate the effects of gibberellic acid application and cold storage on harvested peaches.ResultsApproximately half of the investigated genes exhibited significant differential expression in response to the treatments. Cellular and developmental process gene ontologies were overrepresented among the differentially regulated genes, whereas sequences in cell death and immune response categories were underrepresented. Gene set enrichment demonstrated a predominant role of cold storage in repressing the transcription of genes associated to cell wall metabolism. In contrast, genes involved in hormone responses exhibited a more complex transcriptional response, indicating an extensive network of crosstalk between hormone signaling and low temperatures. Time course transcriptional analyses demonstrate the large contribution of gene expression regulation on the biochemical changes leading to woolliness in peach.ConclusionOverall, our results provide insights on the mechanisms controlling the complex phenotypes associated to postharvest textural changes in peach and suggest that hormone mediated reprogramming previous to pit hardening affects the onset of chilling injuries.

【 授权许可】

CC BY   
© Pegoraro et al. 2015