The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell

BMC Plant Biology

Research Article

Tingting Ning¹  Daiming Jiang¹  Jianlei Pang¹  Daichang Yang¹  Gaoyuan Song¹  Zhibin Guo¹  Zhenwei Liu¹  Yunfang Sun¹ 

[1] State Key Laboratory of Hybrid Rice and College of Life Sciences, Wuhan University, Luojia Hill, 430072, Wuhan, Hubei Province, China;

关键词: OsSec18;    Os60sP0(P1-P2) complex;    Vacuole fusion;    Rice endosperm;   

DOI  :  10.1186/s12870-014-0324-1

 received in 2014-07-21, accepted in 2014-11-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSec18p/N-ethylmaleimide-sensitive factor (NSF) is a conserved eukaryotic ATPase, which primarily functions in vesicle membrane fusion from yeast to human. However, the function of the OsSec18 gene, a homologue of NSF in rice, remains unknown.ResultsIn the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast. Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies. Further examination revealed that OsSec18 presented as a 290-kDa complex in rice endosperm cells. Moreover, Os60sP0 was identified a component of this complex, demonstrating that the OsSec18 complex contains another complex of P0(P1-P2)2 in rice endosperm cells. Furthermore, we determined that the N-terminus of OsSec18 can interact with the N- and C-termini of Os60sP0, whereas the C-terminus of OsSec18 can only interact with the C-terminus of Os60sP0.ConclusionOur results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell.

【 授权许可】

Unknown   
© Sun et al.; licensee BioMed Central Ltd. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.