A meta-analysis of genome-wide association studies of follicular lymphoma

BMC Genomics

Research Article

Henrik Hjalgrim¹  Paige M Bracci²  Keith Humphreys³  Hatef Darabi³  Jianjun Liu⁴  Jia-Nee Foo⁴  Fenna CM Sillé⁵  Christine F Skibola⁵  Sylvia Sanchez⁵  Jacques Riby⁵  Lucia Conde⁵  Karin E Smedby⁶ 

[1] Department of Epidemiology Research, Statens Serum Institut, Copenhagen, Denmark;Department of Epidemiology and Biostatistics, University of California, 94143, San Francisco, CA, USA;Dept of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden;Human Genetics, Genome Institute of Singapore, A*STAR, 138673, Singapore;School of Public Health, Division of Environmental Health Sciences, University of California, 94720, Berkeley, CA, USA;Unit of Clinical Epidemiology, Dept of Medicine Solna, Karolinska Institutet, Stockholm, Sweden;

关键词: Follicular lymphoma (FL);    Genome-wide association studies (GWAS);    Human leukocyte antigen (HLA);    Meta-analysis;   

DOI  :  10.1186/1471-2164-13-516

 received in 2012-06-12, accepted in 2012-09-25,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundB-cell non-Hodgkin lymphoma represents a diverse group of hematological malignancies, of which follicular lymphoma (FL) is one of the most common subtypes. Family and epidemiological studies suggest an important genetic role in the etiology of FL. In recent genome-wide association studies (GWAS) of FL, several genetic susceptibility loci have been identified on chromosome 6p21.33 (rs6457327) and 6p21.32 (rs10484561, rs2647012) in the human leukocyte antigen class I and class II regions. To identify new genetic variants and further elucidate the genetic basis of FL, a meta-analysis was performed of the top 1000 SNPs associated with FL risk from two GWAS in the US, Denmark and Sweden (592 cases, 1541 controls), with independent validation in 107 cases and 681 controls.Resultsrs9275517 and rs3117222 in the HLA class II region were validated and inversely associated with FL risk (rs9275517: OR = 0.63, 95% CI = 0.55-0.73, p = 4.03 × 10-11; rs3117222: OR = 0.66, 95% CI = 0.57-0.77, p = 1.45 × 10-7). rs9275517, which is in high linkage disequilibrium with rs2647012 (r2 = 0.9), was no longer associated with FL after conditioning on rs2647012. The rs3117222 association was independent of established FL SNPs, but not of the HLA-DPB1*0301 allele. Using publicly available gene expression profiles with matching genotype information, we found that rs3117222 also was significantly correlated with increased HLA-DPB1 expression.ConclusionsBy performing a meta-analysis of two GWAS of FL, we further validated the relevance of HLA-DPB1*0301 as a protective allele in the pathogenesis of FL. Moreover, the protective rs3117222 A allele correlated with increased levels of HLA-DPB1, suggesting a possible disease mechanism involving HLA-DPB1 expression regulation. Our results add further support to the major role of HLA genetic variation in the pathogenesis of FL.

【 授权许可】

CC BY   
© Skibola et al.; licensee BioMed Central Ltd. 2012