Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey

BMC Complementary and Alternative Medicine

Research Article

Cristovão Alves da Costa¹  Renah Boanerges de Queiroz Pimentel²  Patrícia Melchionna Albuquerque³  Sergio Duvoisin Junior³ 

[1] Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, 69.060-000, Manaus, AM, Brazil;Universidade do Estado do Amazonas, Programa de Pós-graduação em Biotecnologia e Recursos Naturais da Amazônia, Av. Carvalho Leal, 1777, 69.065-170, Manaus, AM, Brazil;Universidade do Estado do Amazonas, Programa de Pós-graduação em Biotecnologia e Recursos Naturais da Amazônia, Av. Carvalho Leal, 1777, 69.065-170, Manaus, AM, Brazil;Universidade do Estado do Amazonas, Escola Superior de Tecnologia, Laboratório de Química Aplicada à Tecnologia, Av. Darcy Vargas, 1200, 69050-020, Manaus, AM, Brazil;

关键词: Honey;    Stingless bee;    Melipona;    Apis;    Antibacterial activity;    Flavonoids;    HPLC;   

DOI  :  10.1186/1472-6882-13-151

 received in 2012-11-03, accepted in 2013-06-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundHoney has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.MethodsHoney samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.ResultsTwo honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.ConclusionsHoney samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth.

【 授权许可】

Unknown   
© Pimentel et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.