Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs

BMC Musculoskeletal Disorders

Research Article

Xia Chen¹  Yongde Peng¹  Dongliang Xu²  Junsong Chen³  Huaidong Song⁴  Shuangxia Zhao⁴ 

[1] Department of Endocrinology and Metabolism, Shanghai General Hospital of Nanjing Medical University, 100 Haining Road, 200080, Shanghai, China;Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, 200080, Shanghai, Shanghai, China;Key Laboratory of Systems Biomedicine(Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, No. 639 zhizaoju Road, Shanghai, China;

关键词: Osteoglycin;    Peroxisome proliferators-activated receptor-γ 2;    Senile osteoporosis;    Adipogenesis;    Osteoblastogenesis;    Mesenchymal stromal cells;   

DOI  :  10.1186/s12891-017-1779-7

 received in 2017-03-02, accepted in 2017-10-11,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSignificant amount of bone mass is lost during the process of aging due to an imbalance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption in bone marrow microenvironment, which leads to net bone loss in the aging population, resulting in the pathogenesis of osteoporosis.MethodsFirstly, differences in proliferative capacity of adipocyte or adipogenic differentiation in mouse mesenchymal stem cells (MMSCs) and senile mouse model-derived bone marrow mesenchymal stem cells (SMMSCs), as well as mRNA expression of OGN and PPARγ2 were observed. Secondly, osteogenic abilities of MMSCs and SMMSCs treated with rosiglitazone (a PPARγ2 agonist) to induce osteogenic changes were observed, and negative correlation of PPARγ2 with OGN was evaluated. Thirdly, the role of SMMSCs in promoting osteogenesis was examined through enhancing expression of OGN; besides, the related mechanism was investigated by means of expression of related adipocyte and osteoblast specific genes.ResultsForced OGN expression by OGN-infected lentivirus could increase expression of Wnt5b, RUNX2, OCN, ALP and Colla1, as well as bone formation, while decreases expression of adipogenesis marker PPARγ2. It resulted in expression inhibition of adipocyte genes such as adipocytic differentiation related genes adipocyte binding protein 2 (aP2) and osteoclast differentiation factor Rankl in bone marrow, giving rise to increased bone mass.ConclusionOGN may plays a significant role in osteoporosis, which may also provide a potential target for therapeutic intervention of senile osteoporosis characterized by altered differentiation of BMSCs into osteoblasts and adipocytes.

【 授权许可】

CC BY   
© The Author(s). 2017