Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea maysL.)

BMC Plant Biology

Research Article

Joachim Eder¹  Birte Darnhofer¹  Gerhard Wenzel²  Imad Zein²  Thomas Lübberstedt³  Uschi Frei³  Everton A Brenner³  Yongsheng Chen³  Jeppe R Andersen⁴  Milena Ouzunova⁵  Yves Barrière⁶ 

[1] Bavarian State Research Center for Agriculture, Vöttinger Str. 38, 85354, Freising-Weihenstephan, Germany;Department of Agronomy and Plant Breeding, Technical University of Munich, Am Hochanger 2, 85354, Freising-Weihenstephan, Germany;Department of Agronomy, Iowa State University, Agronomy Hall, 50011, Ames, IA, USA;Department of Genetics and Biotechnology, University of Aarhus, Research Center, 4200, Flakkebjerg, Slagelse, Denmark;KWS Saat AG, Grimsehlstr 31, 37555, Einbeck, Germany;Unite' de Ge'ne'tique et d'Ame'lioration des Plantes Fourrage'res, INRA, 86600, Route de Saintes, Lusignan, France;

关键词: Inbred Line;    Polymorphic Site;    Neutral Detergent Fiber;    Cinnamyl Alcohol Dehydrogenase;    COMT Gene;   

DOI  :  10.1186/1471-2229-10-27

 received in 2009-05-12, accepted in 2010-02-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundOMT (O-methyltransferase) genes are involved in lignin biosynthesis, which relates to stover cell wall digestibility. Reduced lignin content is an important determinant of both forage quality and ethanol conversion efficiency of maize stover.ResultsVariation in genomic sequences coding for COMT, CCoAOMT1, and CCoAOMT2 was analyzed in relation to stover cell wall digestibility for a panel of 40 European forage maize inbred lines, and re-analyzed for a panel of 34 lines from a published French study. Different methodologies for association analysis were performed and compared. Across association methodologies, a total number of 25, 12, 1, 6 COMT polymorphic sites were significantly associated with DNDF, OMD, NDF, and WSC, respectively. Association analysis for CCoAOMT1 and CCoAOMT2 identified substantially fewer polymorphic sites (3 and 2, respectively) associated with the investigated traits. Our re-analysis on the 34 lines from a published French dataset identified 14 polymorphic sites significantly associated with cell wall digestibility, two of them were consistent with our study. Promising polymorphisms putatively causally associated with variability of cell wall digestibility were inferred from the total number of significantly associated SNPs/Indels.ConclusionsSeveral polymorphic sites for three O-methyltransferase loci were associated with stover cell wall digestibility. All three tested genes seem to be involved in controlling DNDF, in particular COMT. Thus, considerable variation among Bm3 wildtype alleles can be exploited for improving cell-wall digestibility. Target sites for functional markers were identified enabling development of efficient marker-based selection strategies.

【 授权许可】

Unknown   
© Brenner et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.