ZFNGenome: A comprehensive resource for locating zinc finger nuclease target sites in model organisms

BMC Genomics

Database

Daniel F Voytas¹  Feng Zhang²  Jessica R Kirkpatrick³  Clark R Coffman³  Deepak Reyon⁴  Drena Dobbs⁴  Jeffry D Sander⁵  J Keith Joung⁵ 

[1] Department of Genetics, Cell Biology, and Development, and Center for Genome Engineering, University of Minnesota, 55455, Minneapolis, MN, USA;Department of Genetics, Cell Biology, and Development, and Center for Genome Engineering, University of Minnesota, 55455, Minneapolis, MN, USA;Cellectis Plant Sciences, 1000 Westgate Drive, 55114, St. Paul, MN, USA;Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA;Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA;Bioinformatics and Computational Biology Graduate Program, Iowa State University, 50011, Ames, IA, USA;Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, 02129, Charlestown, MA, USA;Department of Pathology, Harvard Medical School, 02115, Boston, MA, USA;

关键词: Zinc Finger;    Zinc Finger Nuclease;    Protein Code Transcript;    Potential Target Site;    Zinc Finger Array;   

DOI  :  10.1186/1471-2164-12-83

 received in 2010-10-08, accepted in 2011-01-28,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundZinc Finger Nucleases (ZFNs) have tremendous potential as tools to facilitate genomic modifications, such as precise gene knockouts or gene replacements by homologous recombination. ZFNs can be used to advance both basic research and clinical applications, including gene therapy. Recently, the ability to engineer ZFNs that target any desired genomic DNA sequence with high fidelity has improved significantly with the introduction of rapid, robust, and publicly available techniques for ZFN design such as the Oligomerized Pool ENgineering (OPEN) method. The motivation for this study is to make resources for genome modifications using OPEN-generated ZFNs more accessible to researchers by creating a user-friendly interface that identifies and provides quality scores for all potential ZFN target sites in the complete genomes of several model organisms.DescriptionZFNGenome is a GBrowse-based tool for identifying and visualizing potential target sites for OPEN-generated ZFNs. ZFNGenome currently includes a total of more than 11.6 million potential ZFN target sites, mapped within the fully sequenced genomes of seven model organisms; S. cerevisiae, C. reinhardtii, A. thaliana, D. melanogaster, D. rerio, C. elegans, and H. sapiens and can be visualized within the flexible GBrowse environment. Additional model organisms will be included in future updates. ZFNGenome provides information about each potential ZFN target site, including its chromosomal location and position relative to transcription initiation site(s). Users can query ZFNGenome using several different criteria (e.g., gene ID, transcript ID, target site sequence). Tracks in ZFNGenome also provide "uniqueness" and ZiFOpT (Zi nc F inger OP EN T argeter) "confidence" scores that estimate the likelihood that a chosen ZFN target site will function in vivo. ZFNGenome is dynamically linked to ZiFDB, allowing users access to all available information about zinc finger reagents, such as the effectiveness of a given ZFN in creating double-stranded breaks.ConclusionsZFNGenome provides a user-friendly interface that allows researchers to access resources and information regarding genomic target sites for engineered ZFNs in seven model organisms. This genome-wide database of potential ZFN target sites should greatly facilitate the utilization of ZFNs in both basic and clinical research.ZFNGenome is freely available at: http://bindr.gdcb.iastate.edu/ZFNGenome or at the Zinc Finger Consortium website: http://www.zincfingers.org/.

【 授权许可】

CC BY   
© Reyon et al; licensee BioMed Central Ltd. 2011