A diverse set of miRNAs responsive to begomovirus-associated betasatellite in Nicotiana benthamiana

BMC Plant Biology

Research Article

Yu Wang¹  Longjiang Fan¹  Chenhai Yan¹  Yang Liu¹  Chu-Yu Ye¹  Xiuling Yang²  Xiuping Lu³  Bingguang Xiao³  Yongping Li³ 

[1] Department of Agronomy & James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China;Department of Agronomy & James D. Watson Institute of Genome Sciences, Zhejiang University, 310058, Hangzhou, China;State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, China;Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan, 653100, Yuxi, China;

关键词: Nicotiana benthamiana;    miRNA;    phasiRNA;    Begomovirus;   

DOI  :  10.1186/1471-2229-14-60

 received in 2013-12-01, accepted in 2014-03-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRoles of microRNAs (miRNAs) and short interfering RNAs (siRNAs) in biotic stress responses, e.g., viral infection, have been demonstrated in plants by many studies. Tomato yellow leaf curl China virus (TYLCCNV) is a monopartite begomovirus that can systemically infect Solanaceae plants, and induces leaf curling, yellowing and enation symptoms when co-inoculated with a betasatellite (TYLCCNB). The released genome sequence of Nicotiana benthamiana provides an opportunity to identify miRNAs and siRNAs responsive to begomovirus-associated betasatellite in N. benthamiana.ResultsmiRNAs were identified in three small RNA libraries generated using RNA isolated from N. benthamiana plants systemically infected with TYLCCNV (Y10A) alone, co-infected with Y10A and its betasatellite TYLCCNB (Y10β) or a TYLCCNB mutant (Y10mβ) that contains a mutated βC1, the sole betasatellite-encoded protein. A total of 196 conserved miRNAs from 38 families and 197 novel miRNAs from 160 families were identified. Northern blot analysis confirmed that expression of species-specific miRNAs was much lower than that of conserved miRNAs. Several conserved and novel miRNAs were found to be responsive to co-infection of Y10A and Y10β but not to co-infection of Y10A and Y10mβ, suggesting that these miRNAs might play a role unique to interaction between Y10β and N. benthamiana. Additionally, we identified miRNAs that can trigger the production of phased secondary siRNAs (phasiRNAs).ConclusionsIdentification of miRNAs with differential expression profiles in N. benthamiana co-infected with Y10A and Y10β and co-infected with Y10A and Y10mβ indicates that these miRNAs are betasatellite-responsive. Our result also suggested a potential role of miRNA-mediated production of phasiRNAs in interaction between begomovirus and N. benthamiana.

【 授权许可】

CC BY   
© Xiao et al.; licensee BioMed Central Ltd. 2014