The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment

BMC Plant Biology

Research Article

Halina Gabryś¹  Olga Sztatelman²  Agnieszka Katarzyna Banaś³  Joanna Grzyb⁴ 

[1] Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland;Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland;Current address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106, Warszawa, Poland;Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland;The Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland;Laboratory of Biological Physics, Institute of Physics, Polish Academy of Sciences, Al. Lotników 32/46, 02-668, Warszawa, Poland;

关键词: Cell death;    Chlorophyll degradation;    Light;    Photosynthesis;    Senescence;    UV-B;   

DOI  :  10.1186/s12870-015-0667-2

 received in 2015-08-10, accepted in 2015-11-17,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundUltraviolet B (UV-B) irradiation can influence many cellular processes. Irradiation with high UV-B doses causes chlorophyll degradation, a decrease in the expression of genes associated with photosynthesis and its subsequent inhibition. On the other hand, sublethal doses of UV-B are used in post-harvest technology to prevent yellowing in storage. To address this inconsistency the effect of short, high-dose UV-B irradiation on detached Arabidopsis thaliana leaves was examined.ResultsTwo different experimental models were used. After short treatment with a high dose of UV-B the Arabidopsis leaves were either put into darkness or exposed to constant light for up to 4 days. UV-B inhibited dark-induced chlorophyll degradation in Arabidopsis leaves in a dose-dependent manner. The expression of photosynthesis-related genes, chlorophyll content and photosynthetic efficiency were higher in UV-B -treated leaves left in darkness. UV-B treatment followed by constant light caused leaf yellowing and induced the expression of senescence-related genes. Irrespective of light treatment a high UV-B dose led to clearly visible cell death 3 days after irradiation.ConclusionsHigh doses of UV-B have opposing effects on leaves depending on their light status after UV treatment. In darkened leaves short UV-B treatment delays the appearance of senescence symptoms. When followed by light treatment, the same doses of UV-B result in chlorophyll degradation. This restricts the potential usability of UV treatment in postharvest technology to crops which are stored in darkness.

【 授权许可】

CC BY   
© Sztatelman et al. 2015