MiR-339-5p inhibits breast cancer cell migration and invasion in vitro and may be a potential biomarker for breast cancer prognosis

BMC Cancer

Research Article

Xiao-nan Wang¹  Qiang Wu²  Chao-qun Wang²  Yan Wang²  Gui-hong Zhang²  Xiao-chun Xu³  Zheng-sheng Wu⁴  Jing-jing Zhao⁵  Nong Zhang⁵  Shan-shan Mao⁵ 

[1] Department of Microbiology and Parasitology, Anhui Medical University, Hefei, Anhui, P.R. China;Department of Pathology, Anhui Medical University, Hefei, Anhui, P.R. China;Department of Pathology, Anhui Medical University, Hefei, Anhui, P.R. China;Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA;Department of Pathology, Anhui Medical University, Hefei, Anhui, P.R. China;Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, P.R. China;Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, P.R. China;

关键词: Breast Cancer;    Breast Cancer Cell;    Breast Cancer Cell Line;    Breast Cancer Tissue;    Benign Breast Disease;   

DOI  :  10.1186/1471-2407-10-542

 received in 2010-07-21, accepted in 2010-10-09,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) play an important role in the regulation of cell growth, differentiation, apoptosis, and carcinogenesis. Detection of their expression may lead to identifying novel markers for breast cancer.MethodsWe profiled miRNA expression in three breast cancer cell lines (MCF-7, MDA-MB-231, and MDA-MB-468) and then focused on one miRNA, miR-339-5p, for its role in regulation of tumor cell growth, migration, and invasion and target gene expression. We then analyzed miR-339-5p expression in benign and cancerous breast tissue specimens.ResultsA number of miRNAs were differentially expressed in these cancer cell lines. Real-time PCR indicated that miR-339-5p expression was downregulated in the aggressive cell lines MDA-MB-468 and MDA-MB-231 and in breast cancer tissues compared with benign tissues. Transfection of miR-339-5p oligonucleotides reduced cancer cell growth only slightly but significantly decreased tumor cell migration and invasion capacity compared with controls. Real-time PCR analysis showed that BCL-6, a potential target gene of miR-339-5p, was downregulated in MDA-MB-231 cells by miR-339-5p transfection. Furthermore, the reduced miR-339-5p expression was associated with an increase in metastasis to lymph nodes and with high clinical stages. Kaplan-Meier analyses found that the patients with miR-339-5p expression had better overall and relapse-free survivals compared with those without miR-339-5p expression. Cox proportional hazards analyses showed that miR-339-5p expression was an independent prognostic factor for breast cancer patients.ConclusionsMiR-339-5p may play an important role in breast cancer progression, suggesting that miR-339-5p should be further evaluated as a biomarker for predicting the survival of breast cancer patients.

【 授权许可】

CC BY   
© Wu et al; licensee BioMed Central Ltd. 2010