Case report: when two and ½ men go camping…

BMC Infectious Diseases

Case Report

Adrian Egli¹  Carl Chrobak²  Veronika Baettig³  Matthias von Rotz⁴  Ulrich Heininger⁵  Alexa Dierig⁵ 

[1] Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland;Clinical Microbiology, University Hospital Basel, Basel, Switzerland;Department of Internal Medicine, University Hospital Basel, Basel, Switzerland;Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland;Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland;Department of Internal Medicine, University Hospital Basel, Basel, Switzerland;Division of Pediatric Infectious Diseases, Children University Hospital Basel, Basel, Switzerland;

关键词: Listeria monocytogenes;    Sepsis;    MALDI-TOF;    Rapid identification;    Typing;    Pulsed field gel electrophoresis;   

DOI  :  10.1186/s12879-017-2213-3

 received in 2016-08-07, accepted in 2017-01-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIn patients, rapid identification of bacterial species may help to guide treatment at early stages. New protocols for the identification directly from positive blood culture flasks significantly helped in the presented case report.Case presentationTwo patients (a father and son) presented with diarrhea, malaise, and fever of 3 to 4 days duration. Blood cultures from both patients cultured short Gram-positive rods. MALDI-TOF based rapid identification protocol direct from positive blood culture identified Listeria monocytogenes as the cause of sepsis and could be confirmed with conventional methods. Listeria monocytogenes was identified 24 h later by conventional biochemical identification methods (VITEK 2). Antibiotic treatment was adjusted early in response to the MALDI-TOF based identification of bacteremia. Pulsed field gel electrophoresis confirmed the suspected relatedness of the father’s and son’s isolates.ConclusionsMALDI-TOF based may provide a rapid identification of bacterial species directly from positive blood culture.

【 授权许可】

CC BY   
© The Author(s). 2017