Contribution of constitutively proliferating precursor cell subtypes to dentate neurogenesis after cortical infarcts

BMC Neuroscience

Research Article

Christoph Redecker¹  Josephine Walter¹  Silke Keiner¹  Julia Oberland¹ 

[1] Hans-Berger-Clinic for Neurology, University Hospital Jena, Erlanger Allee 101, D - 07747, Jena, Germany;

关键词: Green Fluorescent Protein;    Glial Fibrillary Acidic Protein;    Dentate Gyrus;    Mature Neuron;    Newborn Neuron;   

DOI  :  10.1186/1471-2202-11-146

 received in 2010-03-26, accepted in 2010-11-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIt is well known that focal ischemia increases neurogenesis in the adult dentate gyrus of the hippocampal formation but the cellular mechanisms underlying this proliferative response are only poorly understood. We here investigated whether precursor cells which constitutively proliferate before the ischemic infarct contribute to post-ischemic neurogenesis. To this purpose, transgenic mice expressing green fluorescent protein (GFP) under the control of the nestin promoter received repetitive injections of the proliferation marker bromodeoxyuridine (BrdU) prior to induction of cortical infarcts. We then immunocytochemically analyzed the fate of these BrdU-positive precursor cell subtypes from day 4 to day 28 after the lesion.ResultsQuantification of BrdU-expressing precursor cell populations revealed no alteration in number of radial glia-like type 1 cells but a sequential increase of later precursor cell subtypes in lesioned animals (type 2a cells at day 7, type 3 cells/immature neurons at day 14). These alterations result in an enhanced survival of mature neurons 4 weeks postinfarct.ConclusionsFocal cortical infarcts recruit dentate precursor cells generated already before the infarct and significantly contribute to an enhanced neurogenesis. Our findings thereby increase our understanding of the complex cellular mechanisms of postlesional neurogenesis.

【 授权许可】

Unknown   
© Keiner et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.