Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA

BMC Immunology

Research Article

Hong Chen¹  Nan Deng²  Andrew Kang²  John M. Stuart³  Yonghui Ma⁴  Yanhong Cao⁵  Karen A. Hasty⁶  Weikuan Gu⁶  Yan Jiao⁷  Xiaoyun Liu⁷ 

[1] Center of integrative research, The first Hospital of Qiqihaer City, 30 Gongyuan Road, Longsha District, 161005, Qiqihaer, Heilongjiang, People’s Republic of China;Department of Medicine, University of Tennessee Health Science Center, 38163, Memphis, TN, USA;Department of Medicine, University of Tennessee Health Science Center, 38163, Memphis, TN, USA;Research Service, Veterans Affairs Medical Center, 1030 Jefferson Avenue, 38104, Memphis, TN, USA;Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center (UTHSC), 38163, Memphis, TN, USA;Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center (UTHSC), 38163, Memphis, TN, USA;Institute of Kaschin-beck Disease, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, 150081, Harbin, China;Key Laboratory of Etiologic Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618104), 150081, Harbin, China;Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center (UTHSC), 38163, Memphis, TN, USA;Research Service, Veterans Affairs Medical Center, 1030 Jefferson Avenue, 38104, Memphis, TN, USA;Mudanjiang Medical College, 157001, Mudanjiang, HeilongJiang, People’s Republic of China;Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center (UTHSC), 38163, Memphis, TN, USA;

关键词: Causal Gene;    Recombinant Inbred;    Congenic Strain;    Congenic Mouse;    Decrease Expression Level;   

DOI  :  10.1186/s12865-016-0163-y

 received in 2016-03-31, accepted in 2016-07-12,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe mouse strain BALB/c deficient in IL-1 receptor antagonist protein (Il-1ra) develops spontaneous arthritis disease (SAD) while the strain DBA/1 IL1rn-/- with the same deficiency does not. Previously, we mapped a QTL on chromosome 1 for SAD and then developed a congenic mouse strain BALB.D1-1-/- that contains the QTL genomic fragment associated with resistance from DBA/1-/- on a BALB/c-/- background. The congenic strain was relatively resistant to spontaneous arthritis and had delayed onset and reduced severity of disease. We obtained whole genome expression profiles from the spleen of the congenic strain BALB.D1-1-/- and four other strains, the wild type BALB/c, DBA/1 and the deficient DBA/1 IL1rn-/- and the BALB/c IL1rn-/-. We then compared the similarities and differences between the congenic strain and the four parental strains. Here we report the selected potential causal genes based on differential expression levels as well as function of genes.ResultsThere is a considerable number of genes that are differentially expressed between the congenic strain and the three parental strains, BALB/c, DBA/1, and DBA/1-/-. However there only a few differentially expressed genes were identified by comparing the congenic strain and the BALB/c-/-strain. These differentially expressed genes are mainly from T-cell receptor beta chain (Tcrb) and interferon-activatable protein (Ifi) genes. These genes are also differentially expressed between congenic strain and BALB/c strains. However, their expression levels in the congenic strain are similar to that in DBA/1 and DBA/1-/-. The expression level of Tcrb-j gene is positively associated with two genes of Ifi gene 200 cluster.ConclusionsDecreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease and with down regulation of expressions of Tcrb genes in the mouse congenic strain. Ifi genes may play an important role in the susceptibility to SAD in mice.

【 授权许可】

CC BY   
© The Author(s). 2016