Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential

BMC Biotechnology

Research Article

Johannes H de Winde¹  Luaine Bandounas¹  Harald J Ruijssenaars²  Nick JP Wierckx³ 

[1] B-Basic, Julianalaan 67, 2628, Delft, BC, The Netherlands;Delft University of Technology, Department of Biotechnology, Julianalaan 67, 2628, Delft, BC, The Netherlands;Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628, Delft, BC, The Netherlands;B-Basic, Julianalaan 67, 2628, Delft, BC, The Netherlands;Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628, Delft, BC, The Netherlands;BIRD Engineering BV, Westfrankelandsedijk 1, 3115, Schiedam, HG, The Netherlands;B-Basic, Julianalaan 67, 2628, Delft, BC, The Netherlands;Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628, Delft, BC, The Netherlands;BIRD Engineering BV, Westfrankelandsedijk 1, 3115, Schiedam, HG, The Netherlands;RWTH Aachen University, Institute of Applied Microbiology, Worringerweg 1, 52056, Aachen, Germany;

关键词: Lignin;    Methylene Blue;    Malachite Green;    Mineral Salt Medium;    Luria Broth;   

DOI  :  10.1186/1472-6750-11-94

 received in 2011-04-29, accepted in 2011-10-13,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTo expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. Enzymes could provide an effective means for lignin depolymerization into products of interest. In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization.ResultsBased on 16S rRNA gene sequencing and phenotypic characterization, the organisms were identified as Pandoraea norimbergensis LD001, Pseudomonas sp LD002 and Bacillus sp LD003. The ligninolytic capability of each of these isolates was assessed by growth on high-molecular weight and low-molecular weight lignin fractions, utilization of lignin-associated aromatic monomers and degradation of ligninolytic indicator dyes. Pandoraea norimbergensis LD001 and Pseudomonas sp. LD002 exhibited best growth on lignin fractions, but limited dye-decolourizing capacity. Bacillus sp. LD003, however, showed least efficient growth on lignin fractions but extensive dye-decolourizing capacity, with a particular preference for the recalcitrant phenothiazine dye class (Azure B, Methylene Blue and Toluidene Blue O).ConclusionsBacillus sp. LD003 was selected as a promising source of novel types of ligninolytic enzymes. Our observations suggested that lignin mineralization and depolymerization are separate events which place additional challenges on the screening of ligninolytic microorganisms for specific ligninolytic enzymes.

【 授权许可】

Unknown   
© Bandounas et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.