Hypoglycemic effects of Trichosanthes kirilowii and its protein constituent in diabetic mice: the involvement of insulin receptor pathway

BMC Complementary and Alternative Medicine

Research Article

Chien-Yun Hsiang¹  Tsai-Chung Li²  Hsin-Yi Lo³  Chia-Cheng Li³  Tin-Yun Ho⁴  Jen-Huai Chiang⁵  Tse-Yen Yang⁶ 

[1] Department of Microbiology, China Medical University, 91 Hsueh-Shih Road, 40402, Taichung, Taiwan;Graduate Institute of Biostatistics, China Medical University, 40402, Taichung, Taiwan;Graduate Institute of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, 40402, Taichung, Taiwan;Graduate Institute of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, 40402, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, 41354, Taichung, Taiwan;Management Office for Health Data, China Medical University Hospital, 40447, Taichung, Taiwan;Molecular and Genomic Epidemiology Center, China Medical University Hospital, 40447, Taichung, Taiwan;

关键词: Trichosanthes kirilowii;    Diabetes;    Hypoglycemia;    Insulin receptor;   

DOI  :  10.1186/s12906-017-1578-6

 received in 2016-06-29, accepted in 2017-01-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundDiabetes is a serious chronic metabolic disorder. Trichosanthes kirilowii Maxim. (TK) is traditionally used for the treatment of diabetes in traditional Chinese medicine (TCM). However, the clinical application of TK on diabetic patients and the hypoglycemic efficacies of TK are still unclear.MethodsA retrospective cohort study was conducted to analyze the usage of Chinese herbs in patients with type 2 diabetes in Taiwan. Glucose tolerance test was performed to analyze the hypoglycemic effect of TK. Proteomic approach was performed to identify the protein constituents of TK. Insulin receptor (IR) kinase activity assay and glucose tolerance tests in diabetic mice were further used to elucidate the hypoglycemic mechanisms and efficacies of TK.ResultsBy a retrospective cohort study, we found that TK was the most frequently used Chinese medicinal herb in type 2 diabetic patients in Taiwan. Oral administration of aqueous extract of TK displayed hypoglycemic effects in a dose-dependent manner in mice. An abundant novel TK protein (TKP) was further identified by proteomic approach. TKP interacted with IR by docking analysis and activated the kinase activity of IR. In addition, TKP enhanced the clearance of glucose in diabetic mice in a dose-dependent manner.ConclusionsIn conclusion, this study applied a bed-to-bench approach to elucidate the hypoglycemic efficacies and mechanisms of TK on clinical usage. In addition, we newly identified a hypoglycemic protein TKP from TK. Our findings might provide a reasonable explanation of TK on the treatment of diabetes in TCM.

【 授权许可】

CC BY   
© The Author(s). 2017