An immunohistochemical, enzymatic, and behavioral study of CD157/BST-1 as a neuroregulator

BMC Neuroscience

Research Article

Tae-Sik Nam¹  Uh-Hyun Kim¹  Katsuhiko Ishihara²  Satoka Kasai³  Shigeru Yokoyama³  Haruhiro Higashida³  Jing Zhong³  Azam Fakhrul³  Chiharu Tsuji³  Tomoko Nishimura³  Mingkun Liang³  Toru Yoshihara³  Naila Al Mahmuda³  Shirin Akther³  Cherepanov Stanislav³  Takahiro Tsuji³  Maria Gerasimenko³  Olga Lopatina⁴  Alla Salmina⁴ 

[1] Department of Biochemistry, Chonbuk National University Medical School, Jeonju, South Korea;Department of Immunology and Molecular Genetics, Kawasaki Medical School, 701-0192, Kurashiki, Okayama, Japan;Research Centre for Child Mental Development, Kanazawa University, 920-8640, Kanazawa, Japan;Research Centre for Child Mental Development, Kanazawa University, 920-8640, Kanazawa, Japan;Department of Biochemistry, Medical, Pharmaceutical and Toxicological Chemistry, Krasnoyarsk State Medical University, 660022, Krasnoyarsk, Russia;

关键词: Autism Spectrum Disorder;    Autism Spectrum Disorder;    Paneth Cell;    Nicotinic Acid Adenine Dinucleotide Phosphate;    CD157 Immunoreactivity;   

DOI  :  10.1186/s12868-017-0350-7

 received in 2016-07-07, accepted in 2017-03-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRecent rodent and human studies provide evidence in support of the fact that CD157, well known as bone marrow stromal cell antigen-1 (BST-1) and a risk factor in Parkinson’s disease, also meaningfully acts in the brain as a neuroregulator and affects social behaviors. It has been shown that social behaviors are impaired in CD157 knockout mice without severe motor dysfunction and that CD157/BST1 gene single nucleotide polymorphisms are associated with autism spectrum disorder in humans. However, it is still necessary to determine how this molecule contributes to the brain’s physiological and pathophysiological functions.MethodsTo gain fresh insights about the relationship between the presence of CD157 in the brain and its enzymatic activity, and aberrant social behavior, CD157 knockout mice of various ages were tested.ResultsCD157 immunoreactivity colocalized with nestin-positive cells and elements in the ventricular zones in E17 embryos. Brain CD157 mRNA levels were high in neonates but low in adults. Weak but distinct immunoreactivity was detected in several areas in the adult brain, including the amygdala. CD157 has little or no base exchange activity, but some ADP-ribosyl cyclase activity, indicating that CD157 formed cyclic ADP-ribose but much less nicotinic acid adenine dinucleotide phosphate, with both mobilizing Ca2+ from intracellular Ca2+ pools. Social avoidance in CD157 knockout mice was rescued by a single intraperitoneal injection of oxytocin.ConclusionsCD157 may play a role in the embryonic and adult nervous systems. The functional features of CD157 can be explained in part through the production of cyclic ADP-ribose rather than nicotinic acid adenine dinucleotide phosphate. Further experiments are required to elucidate how the embryonic expression of CD157 in neural stem cells contributes to behaviors in adults or to psychiatric symptoms.

【 授权许可】

CC BY   
© The Author(s) 2017