Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches

BMC Genomics

Research Article

Krishnareddy Bayyareddy¹  Michael J Adang²  Guillaume Tetreau³  Jean-Philippe David³  Margot Paris³  Renaud Stalinski³  Muhammad A Riaz³  Laurence Després³  Christopher M Jones⁴ 

[1] Department of Entomology, University of Georgia, 30602-2603, Athens, GA, USA;Department of Entomology, University of Georgia, 30602-2603, Athens, GA, USA;Department of Biochemistry and Molecular Biology, University of Georgia, 30602-2603, Athens, GA, USA;Laboratoire d’Ecologie Alpine, LECA-UMR 5553, Université de Grenoble 1, BP 53, 38041, Grenoble, cedex 09, France;Vector Group, Liverpool School of Tropical Medicine, L3 5QA, Liverpool, UK;

关键词: Aedes aegypti;    Bacillus thuringiensis israelensis;    DIGE;    Microarray;    RT-qPCR;    Resistance;    Transcriptomics;    Proteomics;    Midgut;    Mosquito;    Candidate genes;   

DOI  :  10.1186/1471-2164-13-248

 received in 2012-03-13, accepted in 2012-05-25,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundBacillus thuringiensis var. israelensis (Bti) is a natural larval mosquito pathogen producing pore-forming toxins targeting the midgut of Diptera larvae. It is used worldwide for mosquito control. Resistance mechanisms of an Aedes aegypti laboratory strain selected for 30 generations with field-collected leaf litter containing Bti toxins were investigated in larval midguts at two levels: 1. gene transcription using DNA microarray and RT-qPCR and 2. differential expression of brush border membrane proteins using DIGE (Differential In Gel Electrophoresis).ResultsSeveral Bti Cry toxin receptors including alkaline phosphatases and N-aminopeptidases and toxin-binding V-ATPases exhibited altered expression levels in the resistant strain. The under-expression of putative Bti- receptors is consistent with Bt-resistance mechanisms previously described in Lepidoptera. Four soluble metalloproteinases were found under-transcribed together with a drastic decrease of metalloproteinases activity in the resistant strain, suggesting a role in resistance by decreasing the amount of activated Cry toxins in the larval midgut.ConclusionsBy combining transcriptomic and proteomic approaches, we detected expression changes at nearly each step of the ingestion-to-infection process, providing a short list of genes and proteins potentially involved in Bti-resistance whose implication needs to be validated. Collectively, these results open the way to further functional analyses to better characterize Bti- resistance mechanisms in mosquitoes.

【 授权许可】

CC BY   
© Tetreau et al.; licensee BioMed Central Ltd. 2012