Anti-inflammatory effects of Neurotoxin-Nna, a peptide separated from the venom of Naja naja atra

BMC Complementary and Alternative Medicine

Research Article

Shuijuan Zhang¹  Yeping Ruan¹  Li Yao¹  Bingbing Zhang¹  Jianyou Guo² 

[1] College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 310053, Hangzhou, P.R, China;Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, 100101, Beijing, P.R, China;

关键词: Neurotoxin-Nna;    Anti-inflammatory;    Antinociceptive;    Tumor necrosis factor alpha;    Interleukin 1 beta;    Nuclear factor kappa B;   

DOI  :  10.1186/1472-6882-13-86

 received in 2012-11-25, accepted in 2013-03-11,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundNeurotoxin-Nna (NT), an analgesic peptide separated from the venom of Naja naja atra, has reported to have an exceptional specificity to block transmission of the nerve impulse by binding to the α- subunit of the nicotinic acetylcholine receptor in the membrane. However, little information is available on the anti-inflammatory effects of NT. Therefore, the anti-inflammatory activity of Neurotoxin-Nna was investigated in this study.MethodsThe anti-inflammatory effects of NT were evaluated by measuring its influence on several crucial factors in inflammatory pathways, including total antioxidant activity, antinociceptive effects in vivo, nuclear factor kappa B (NF-κB), polymorphonuclear cells (PMN), inducible nitric oxide synthase (iNOS), adhesion molecule (ICAM-1) and tactile hyperalgesia.ResultsNT treatment decreased the levels of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β). NT treatment decreased the total antioxidant status (TAOS) and reduced CFA-induced tactile hyperalgesia in a dose-dependent manner. NT significantly inhibited regulation of NF-kappaB activation and the production of IL-1β, TNF-α, iNOS and CAM-1. Moreover, NT suppressed infiltration of PMN.ConclusionsOur results showed that NT reduced CFA-induced tactile hyperalgesia through inhibition inflammatory pathways in experimental inflammatory rats.

【 授权许可】

CC BY   
© Ruan et al.; licensee BioMed Central Ltd. 2013