Amelioration of azoxymethane induced-carcinogenesis by reducing oxidative stress in rat colon by natural extracts

BMC Complementary and Alternative Medicine

Research Article

Sardar A Farooq¹  Ahmed Al-Alawi²  Amani S Al-Rawahi²  Mostafa I Waly²  Mohammad S Rahman²  Marwa Al Riyami³  Mohamed A Al-Kindi³  Halima K Al-Issaei³ 

[1] Department of Biology, College of Science, Sultan Qaboos University, P. O. Box 34–123, Al-Khod, Muscat, Oman;Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, P. O. Box 34–123, Al-Khod, Muscat, Oman;Pathology Department, College of Medicine and Health Sciences, Sultan Qaboos University, P. O. Box 34–123, Al-Khod, Muscat, Oman;

关键词: Azoxymethane;    Oxidative stress;    Colon cancer;   

DOI  :  10.1186/1472-6882-14-60

 received in 2013-04-11, accepted in 2014-02-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAzoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon.MethodsEighty Sprague–Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant crypt foci (ACF) development, genotoxicity (induced micronuclei (MN) cells enumeration), and glutathione and lipid peroxidation.ResultsOur results showed that AOM-induced ACF development and pathological changes in the colonic mucosal tissues, increased bone marrow MN cells and oxidative stress (glutathione depletion, lipid peroxidation) in rat colonic cells. The concomitant treatment of AOM with PomPE, PapPE or SE significantly ameliorated the cytotoxic effects of AOM.ConclusionsThe results of this study provide in-vivo evidence that PomPE, PapPE and SE reduced the AOM-induced colon cancer in rats, through their potent anti-oxidant activities.

【 授权许可】

Unknown   
© Waly et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.