Glyma11g13220, a homolog of the vernalization pathway gene VERNALIZATION 1 from soybean [Glycine max (L.) Merr.], promotes flowering in Arabidopsis thaliana

BMC Plant Biology

Research Article

Haicui Suo¹  Hai Nian²  Rong Yi²  Jing Lü²  Qibin Ma² 

[1] The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China;The Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China;Guangdong Provincial Key Laboratory of Crop Genetics and Improvement, Guangzhou, China;The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China;The Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, China;The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China;

关键词: Shoot Apex;    Early Flowering;    Vernalization Response;    Regulate Flowering Time;    Vernalization Pathway;   

DOI  :  10.1186/s12870-015-0602-6

 received in 2015-03-19, accepted in 2015-09-04,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe precise timing of flowering is fundamental to successful reproduction, and has dramatic significance for crop yields. Although prolonged low temperatures are not required for flowering induction in soybean, vernalization pathway genes have been retained during the evolution of this species. Little information is currently available in regarding these genes in soybean.ResultsWe were able to detect the expression of Glyma11g13220 in different organs at all monitored developmental stages in soybean. Glyma11g13220 expression was higher in leaves and pods than in shoot apexes and stems. In addition, Glyma11g13220 was responsive to photoperiod and low temperature in soybean. Furthermore, Glyma11g13220 was found to be a nuclear-localized protein. Over-expression of Glyma11g13220 in an Arabidopsis Columbia-0 (Col-0) background resulted in early flowering. Quantitative real-time PCR analysis revealed that transcript levels of flower repressor FLOWERING LOCUS C (FLC), and FD decreased significantly in transgenic Arabidopsis compared with wild-type Col-0, while the expression of VERNALIZATION INSENSITIVE 3 (VIN3) and FLOWERING LOCUS T (FT) noticeably increased.ConclusionsOur results suggest that Glyma11g13220, a homolog of Arabidopsis VRN1, is a functional protein. Glyma11g13220, which is responsive to photoperiod and low temperature in soybean, may participate in the vernalization pathway in Arabidopsis and help regulate flowering time. Arabidopsis VRN1 and Glyma11g13220 exhibit conserved as well as diverged functions.

【 授权许可】

CC BY   
© Lü et al. 2015