Copy Number Amplification of the PIK3CA Gene Is Associated with Poor Prognosis in Non-lymph node metastatic Head and Neck Squamous Cell Carcinoma

BMC Cancer

Research Article

Toshihito Suda¹  Hiroshi Moriyama¹  Mamoru Yoshikawa¹  Takakuni Kato¹  Takanori Hama²  Yuki Yuza³  Shu Kondo⁴  Mitsuyoshi Urashima⁵ 

[1] Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, 105-8461, Tokyo, Minato-ku, Japan;Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, 105-8461, Tokyo, Minato-ku, Japan;Division of Molecular Epidemiology, Jikei University School of Medicine, Tokyo, Minato-ku, Japan;Department of Pediatrics, Jikei University School of Medicine, Tokyo, Minato-ku, Japan;Division of Invertebrate Genetics, National Institute of Genetics, Mishima, Japan;Division of Molecular Epidemiology, Jikei University School of Medicine, Tokyo, Minato-ku, Japan;Department of Pediatrics, Jikei University School of Medicine, Tokyo, Minato-ku, Japan;

关键词: PIK3CA;    KRAS;    BRAF;    Copy number analysis;    Prognostic Factor;   

DOI  :  10.1186/1471-2407-12-416

 received in 2012-01-27, accepted in 2012-09-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDeregulation of the EGFR signaling pathway is one of the most frequently observed genetic abnormalities that drives cancer development. Although mutations in the downstream components of the EGFR signaling pathway, including KRAS, BRAF and PIK3CA, have been reported in numerous cancers, extensive mutation and copy number analysis of these genes in clinical samples has not been performed for head and neck squamous cell carcinoma (HNSCC).MethodsWe examined the mutations and copy number alterations of KRAS, BRAF and PIK3CA in 115 clinical specimens of HNSCC obtained from surgically treated patients.We used DNA sequencing to detect mutations and the copy number changes were evaluated by qPCR and array comparative genomic hybridization (CGH) analysis.ResultsWe examined the mutations and copy number alterations of KRAS, BRAF and PIK3CA in 115 clinical specimens of HNSCC obtained from surgically treated patients. We identified 3 mutations (2.6%) in K-RAS and 3 mutations (2.6%) in PIK3CA. Copy number amplification was found in 37 cases (32.2%) for PIK3CA, 10 cases (8.7%) for K-RAS and 2 cases (1.7%) for BRAF. Kaplan-Meier survival analysis revealed that copy-number amplification of PIK3CA was markedly associated with cancer relapse in patients without lymph node metastasis. (Log-rank test, p = 0.026)ConclusionsCopy number amplification of the PIK3CA gene is associated with poor prognosis in HNSCC patients without lymph node metastasis. The PIK3CA copy number status will serve as a marker of poor prognosis in patients with HNSCC.

【 授权许可】

Unknown   
© Suda et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.