Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean

BMC Plant Biology

Research Article

Gary J Muehlbauer¹  Bindu Joseph²  James E Specht³  Brian W Diers⁴  Wayne W Xu⁵  Zheng Jin Tu⁵  Gregory D May⁶  Andrew D Farmer⁶  Steven B Cannon⁷  Randy C Shoemaker⁷  Michelle A Graham⁷  Nathan Weeks⁷  Yung-Tsi Bolon⁸  Carroll P Vance⁹ 

[1] Department of Agronomy and Plant Genetics, University of Minnesota, 55108, St Paul, MN, USA;Department of Agronomy, Iowa State University, 50011, Ames, IA, USA;Department of Agronomy, University of Nebraska, 68583, Lincoln, NE, USA;Department of Crop Sciences, University of Illinois, 1101 West Peabody Dr, 61801, Urbana, IL, USA;Minnesota Supercomputing Institute, University of Minnesota, 55455, Minneapolis, MN, USA;National Center for Genome Resources, 87505, Santa Fe, NM, USA;United States Department of Agriculture-Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, 50011, Ames, IA, USA;United States Department of Agriculture-Agricultural Research Service, Plant Research Unit, 55108, St Paul, MN, USA;United States Department of Agriculture-Agricultural Research Service, Plant Research Unit, 55108, St Paul, MN, USA;Department of Agronomy and Plant Genetics, University of Minnesota, 55108, St Paul, MN, USA;

关键词: Quantitative Trait Locus;    Seed Development;    Seed Protein;    Quantitative Trait Locus Region;    Soybean Genome;   

DOI  :  10.1186/1471-2229-10-41

 received in 2009-10-15, accepted in 2010-03-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content.ResultsA soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix® Soy GeneChip and high-throughput Illumina® whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region.ConclusionsThis study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.

【 授权许可】

Unknown   
© Bolon et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.