In vitro and ex-vivo cellular antioxidant protection and cognitive enhancing effects of an extract of Polygonum minus Huds (Lineminus™) demonstrated in a Barnes Maze animal model for memory and learning

BMC Complementary and Alternative Medicine

Research Article

Annie George¹  Hoi Jin Wong¹  Chee Perng Ng²  Matthew O’Callaghan²  Gitte S Jensen³ 

[1] Biotropics Malaysia Berhad, Lot 21, Jalan U1/19, Section U1, Hicom-Glenmarie Industrial Park, 40150, Shah Alam, Selangor, Malaysia;Cerca Insights Sdn Bhd (NCIA Technology Development Center) Level 2, Plot No.36 Hilir Sungai Keluang, Bayan Lepas Industrial EstatePhase IV, 11900, Bayan Lepas, Penang, Malaysia;NIS Labs, 1437 Esplanade, 97601, Klamath Falls, OR, USA;

关键词: Scopolamine;    Antioxidant;    Barnes maze;    Polygonum minus;    Cognition;   

DOI  :  10.1186/1472-6882-14-161

 received in 2013-12-23, accepted in 2014-05-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPolygonum minus Huds.is a culinary flavouring that is common in South East Asian cuisine and as a remedy for diverse maladies ranging from indigestion to poor eyesight. The leaves of this herb have been reported to be high in antioxidants. Flavonoids which have been associated with memory, cognition and protection against neurodegeneration were found in P. minus.MethodThis study examined a P. minus aqueous extract (Lineminus™) for its antioxidant activity using the Oxygen Radical Absorbance Capacity (ORAC) assay, the ex vivo Cellular Antioxidant Protection of erythrocytes (CAP-e) assays and for potential anticholinesterase activity in vitro. Cognitive function and learning of Lineminus™ was evaluated using scopolamine induced cognition deficits in a Barnes maze, rodent model of cognition.ResultsThe extract displayed in vitro antioxidant activity with a total ORAC value of 16,964 μmole TE/gram. Cellular antioxidant protection from free radical damage using the CAP-e assay, with an IC50 of 0.58 g/L for inhibition of cellular oxidative damage, was observed. The extract inhibited cholinesterase activity with an IC50 of 0.04 mg/ml with a maximum inhibition of 68%. In a rodent model of cognition using scopolamine induced cognition deficits in the Barnes maze, the extract attenuated scopolamine induced disruptions in learning at the higher dose of 100 mg/kg.ConclusionThese data shows that P. minus possesses antioxidant and anticholinesterase activity and demonstrated enhanced cognition in vivo. The data suggest neuroprotective properties of the extract.

【 授权许可】

CC BY   
© George et al.; licensee BioMed Central Ltd. 2014