Histone acetyltransferases in rice (Oryza sativaL.): phylogenetic analysis, subcellular localization and expression

BMC Plant Biology

Research Article

Keqiang Wu¹  Jun Duan²  Jianxia Zhang²  Jinhui Zhao³  Wei Zhang³  Xia Liu⁴  Ming Luo⁵  Lining Tian⁶ 

[1] Institute of Plant Biology,National Taiwan University, 106, Taipei, Taiwan;South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China;South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China;Graduate School of the Chinese Academy of Sciences, 100039, Beijing, China;South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China;Graduate School of the Chinese Academy of Sciences, 100039, Beijing, China;Southern Crop Protection and Food Research Centre,Agriculture and Agri-Food Canada, N5V 4T3, London, ON, Canada;South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China;Institute of Plant Biology,National Taiwan University, 106, Taipei, Taiwan;Southern Crop Protection and Food Research Centre,Agriculture and Agri-Food Canada, N5V 4T3, London, ON, Canada;

关键词: Histone acetyltransferase;    Hormone;    Phylogenetic tree;    Subcellular localization;    Rice;    Stress;   

DOI  :  10.1186/1471-2229-12-145

 received in 2011-11-23, accepted in 2012-08-10,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundHistone acetyltransferases (HATs) play an important role in eukaryotic transcription. Eight HATs identified in rice (OsHATs) can be organized into four families, namely the CBP (OsHAC701, OsHAC703, and OsHAC704), TAFII250 (OsHAF701), GNAT (OsHAG702, OsHAG703, and OsHAG704), and MYST (OsHAM701) families. The biological functions of HATs in rice remain unknown, so a comprehensive protein sequence analysis of the HAT families was conducted to investigate their potential functions. In addition, the subcellular localization and expression patterns of the eight OsHATs were analyzed.ResultsOn the basis of a phylogenetic and domain analysis, monocotyledonous CBP family proteins can be subdivided into two groups, namely Group I and Group II. Similarly, dicotyledonous CBP family proteins can be divided into two groups, namely Group A and Group B. High similarities of protein sequences, conserved domains and three-dimensional models were identified among OsHATs and their homologs in Arabidopsis thaliana and maize. Subcellular localization predictions indicated that all OsHATs might localize in both the nucleus and cytosol. Transient expression in Arabidopsis protoplasts confirmed the nuclear and cytosolic localization of OsHAC701, OsHAG702, and OsHAG704. Real-time quantitative polymerase chain reaction analysis demonstrated that the eight OsHATs were expressed in all tissues examined with significant differences in transcript abundance, and their expression was modulated by abscisic acid and salicylic acid as well as abiotic factors such as salt, cold, and heat stresses.ConclusionsBoth monocotyledonous and dicotyledonous CBP family proteins can be divided into two distinct groups, which suggest the possibility of functional diversification. The high similarities of protein sequences, conserved domains and three-dimensional models among OsHATs and their homologs in Arabidopsis and maize suggested that OsHATs have multiple functions. OsHAC701, OsHAG702, and OsHAG704 were localized in both the nucleus and cytosol in transient expression analyses with Arabidopsis protoplasts. OsHATs were expressed constitutively in rice, and their expression was regulated by exogenous hormones and abiotic stresses, which suggested that OsHATs may play important roles in plant defense responses.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.