Comparative proteomics analysis of teleost intermuscular bones and ribs provides insight into their development

BMC Genomics

Research Article

Wei-Min Wang¹  Chun-Hong Nie²  Shi-Ming Wan²  Ze-Xia Gao³  Tomislav Treer⁴  Tea Tomljanovic⁴  Chung-Der Hsiao⁵ 

[1] College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, 430070, Wuhan, Hubei, China;College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Collaborative Innovation Center for Healthy Freshwater Aquaculture of Hubei Province, 430070, Wuhan, China;College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Collaborative Innovation Center for Healthy Freshwater Aquaculture of Hubei Province, 430070, Wuhan, China;Hubei Provincial Engineering Laboratory for Pond Aquaculture, 430070, Wuhan, Hubei, China;Department for Fisheries, Beekeeping, Game management and Special Zoology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia;Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, Taiwan;

关键词: Megalobrama amblycephala;    Intermuscular bones;    Ribs;    iTRAQ proteomics;    MRM validation;    Growth and differentiation;   

DOI  :  10.1186/s12864-017-3530-z

 received in 2016-09-17, accepted in 2017-01-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIntermuscular bones (IBs) and ribs both are a part of skeletal system in teleosts, but with different developing process. The chemical composition of fish IBs and ribs as well as the underlying mechanism about their development have not been investigated. In the present study, histological structures showed that one bone cavity containing osteoclasts were existed in ribs, but not in IBs of Megalobrama amblycephala. We constructed the first proteomics map for fish bones including IBs and ribs, and identified the differentially expressed proteins between IBs and ribs through iTRAQ LC-MS/MS proteomic analysis.ResultsThe proteins extracted from IBs and ribs at 1- to 2-year old M. amblycephala were quantified 2,342 proteins, with 1,451 proteins annotated with GO annotation in biological processes, molecular function and cellular component. A number of bone related proteins as well as pathways were identified in the study. A total of 93 and 154 differently expressed proteins were identified in comparison groups of 1-IB-vs-1-Rib and 2-IB-vs-2-Rib, which indicated the obvious differences of chemical composition between these two bone tissues. The two proteins (vitronectin b precursor and matrix metalloproteinase-2) related to osteoclasts differentiation were significantly up-regulated in ribs compared with IBs (P < 0.05), which was in accordance with the results from histological structures. In comparison groups of 1-IB-vs-2-IB and 1-Rib-vs-2-Rib, 33 and 51 differently expressed proteins were identified and the function annotation results showed that these proteins were involved in regulating bone development and differentiation. Subsequently, 11 and 13 candidate proteins in comparison group of 1-IB-vs-1-Rib and 1-IB-vs-2-IB related to bone development were validated by MRM assays.ConclusionsOur present study suggested the different key proteins involved in the composition of fish ribs and IBs as well as their growth development. These findings could provide important clues towards further understanding of fish skeletal system and the roles of proteins playing in regulating diverse biological processes in fish.

【 授权许可】

CC BY   
© The Author(s). 2017