Establishment and characterization of new cell lines of anaplastic pancreatic cancer, which is a rare malignancy: OCUP-A1 and OCUP-A2

BMC Cancer

Research Article

Akihiro Murata¹  Tsuyoshi Hasegawa²  Bunzo Nakata³  Go Ohira⁴  Kotaro Miura⁴  Ryosuke Amano⁴  Masakazu Yashiro⁴  Sadaaki Yamazoe⁴  Masaichi Ohira⁴  Kenjiro Kimura⁴  Kohei Nishio⁴  Kosei Hirakawa⁴ 

[1] Department of Hepato-Biliary Pancreatic Surgery, Osaka City General Medical Center, 13-22, 2-chome, Miyakojimahondori, Miyakojima-ku, 534-0021, Osaka city, Osaka, Japan;Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School, Harry T. Lester Hall 421 651 Colley Avenue,, 23501, Norfolk, VA, USA;Department of Surgery, Kashiwara Municipal Hospital, 1-chome, 7-9, Hozenji, 582-0005, Kashiwara city, Osaka, Japan;Department of Surgical Oncology, Osaka City University Graduate School of Medicine, 4-3, 1-chome, Asahimachi, Abeno-ku, 545-8585, Osaka city, Osaka, Japan;

关键词: Anaplastic pancreatic cancer;    Human cell line;    Rare malignancy;   

DOI  :  10.1186/s12885-016-2297-y

 received in 2015-10-15, accepted in 2016-03-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAnaplastic pancreatic cancer (APC) cell lines have been scarcely established.MethodsThe morphology, gene expressions, karyotyping and epithelial-mesenchymal transition markers of newly established APC cell lines OCUP-A1 and OCUP-A2 were analyzed. Their abilities of proliferation under normoxia and hypoxia, migration and invasion were compared to 4 commercially available pancreatic ductal adenocarcinoma (PDA) cell lines. Their induction of angiogenesis, stem-like cell population and subcutaneous tumor growth in nude mice were estimated, comparing 2 PDA cell lines examined here.ResultsOCUP-A1 and OCUP-A2 cells continuously grew with spindle and polygonal shapes, respectively. Gene analysis revealed 9 gene mutations including KRAS and TP53. Karyotyping clarified numerical structural abnormalities in both cells. Loss of E-cadherin and expression of vimentin in both cell lines were observed. The doubling time of both cell lines was approximately 20 h. Proliferation, migration and invasion abilities were not notable compared to other PDA cell lines. However stem-like cell population of both cell lines was superior to a part of PDA cell lines. Moreover OCUP-A1 showed stronger hypoxia tolerance and induction of angiogenesis than other PDA cell lines. The tumorigenicity in vivo of OCUP-A2 was stronger than conventional PDA cell lines.ConclusionsThe OCUP-A1 and OCUP-A2 cell lines of rare malignancies might be useful for investigating the biology of pancreatic cancer.

【 授权许可】

CC BY   
© Miura et al. 2016