Organic silicon protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide effects

BMC Complementary and Alternative Medicine

Research Article

Maria Pilar González¹  José J Merino¹  Maria Isabel Sánchez-Reus¹  Sara Bastida²  Francisco J Sánchez-Muniz²  Juana Benedí³  Alba Garcimartín⁴ 

[1]Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain

[2]Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain

[3]Depatamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain

[4]Depatamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain

[5]Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, Spain

关键词: Silicon;    Cellular death;    LDH;    ROS;    Caspase-3,-8,-9;    Neuroprotection;   

DOI  :  10.1186/1472-6882-14-384

 received in 2014-06-30, accepted in 2014-09-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHydrogen peroxide (H2O2) is a toxic agent that induces oxidative stress and cell death. Silicon (Si) is a biological element involved in limiting aluminium (Al) absorption with possible preventive effects in Alzheimer’s disease. However, Si has not yet been associated with other neuroprotective mechanisms.MethodsThe present experiments evaluated in the SH-SY5Y human neuroblastoma cell line the possible role of different Si G5 (50-1000 ng/mL) concentrations in preventing cellular death induced by H2O2 (400 μM, 24 hours).ResultsOur findings showed that H2O2 promoted cell death in the human SH-SY5Y cell cultures and this could be prevented by Si treatment. The loss in cell viability mediated by H2O2 was due to an apoptotic and necrotic process. Apoptotic death was incurred by regulating caspase-8 activity in the extrinsic pathway. The apoptotic and necrotic cell death induced by H2O2 was almost totally reversed by Si (50-500 ng/mL), indicating that it down-regulates both processes in H2O2 treated cells.ConclusionsAccording to our data, Si is able to increase SH-SY5Y cell survival throughout partially blocking cellular damage related to oxidative stress through a mechanism that would affect H2O2/ROS elimination.

【 授权许可】

CC BY   
© Garcimartín et al.; licensee BioMed Central Ltd. 2014