Comparison of investigation methods of heat injury in grapevine (Vitis) and assessment to heat tolerance in different cultivars and species

BMC Plant Biology

Research Article

Guojie Liu¹  Hongguo Xu²  Lijun Wang³  Wei Duan³  Shaohua Li⁴  Guotian Liu⁵  Bofang Yan⁵ 

[1] College of Agronomy and Biotechnology, China Agricultural University, 100193, Beijing, China;College of Agronomy and Biotechnology, China Agricultural University, 100193, Beijing, China;Key Laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, the Chinese Academy of Sciences, 100093, Beijing, People's Republic of China;Key Laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, the Chinese Academy of Sciences, 100093, Beijing, People's Republic of China;Key Laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, the Chinese Academy of Sciences, 100093, Beijing, People's Republic of China;Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, 430074, Wuhan, People's Republic of China;Key Laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, the Chinese Academy of Sciences, 100093, Beijing, People's Republic of China;University of Chinese Academy of Sciences, 100049, Beijing, People's Republic of China;

关键词: Heat Stress;    Leaf Disc;    Electrolyte Leakage;    Heat Tolerance;    PSII Reaction Center;   

DOI  :  10.1186/1471-2229-14-156

 received in 2014-04-13, accepted in 2014-05-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundIn the context of global climate change, heat stress is becoming an increasingly important constraint on grapevine growth and berry quality. There is a need to breed new grape cultivars with heat tolerance and to design effective physiological defenses against heat stress. The investigation of heat injury to plants or tissues under high temperature is an important step in achieving these goals. At present, evaluation methods for heat injury include the gas exchange parameters of photosynthesis, membrane thermostability, chlorophyll content etc.; however, these methods have obvious disadvantages, such as insensitivity, inconvenience and delayed information. An effective and convenient method for investigating the heat injury of grapevine must be developed.ResultsIn this study, an investigation protocol for a critical temperature (47°C) and heat treatment time (40 min) was developed in detached grape leaves. Based on the results, we found that the OJIP test was superior to measuring electrolyte leakage or photosynthetic O2 evolution for investigating the heat injury of three cultivars of grapevine. Heat tolerance of 47 grape species and cultivars was evaluated through investigating heat injury using the OJIP test. Moreover, the electron transport chain (donor side, acceptor side and reaction center) of PSII in photosynthesis was further investigated.ConclusionsThe OJIP test was a rapid, sensitive and convenient method for investigating heat injury in grapevine. An analysis of PSII function using this method indicated that the acceptor side was less sensitive to heat than was the donor side or the reaction center in grape leaves. Among the 47 taxa evaluated (cultivars, hybrids, and wild species), heat tolerance varied largely in each genotype group: most wild species and hybrids between V. labrusca and V. vinifera had relatively strong heat tolerance, but most cultivars from V. vinifera had relatively weak heat tolerance.

【 授权许可】

Unknown   
© Xu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.