In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection

BMC Immunology

Research Article

Marcel Marín-Villa¹  Yara M Traub-Cseko¹  Carlos R Alves²  Franklin S Silva²  Bernardo AS Pereira²  Karina M Rebello²  Thereza CB Andrade³  Álvaro L Bertho⁴  Ernesto R Caffarena⁵ 

[1] Laboratório de Biologia Molecular de Parasitas e Vetores, IOC, Fiocruz, Rio de Janeiro, RJ, Brasil;Laboratório de Biologia Molecular e Doenças Endêmicas, IOC,Fiocruz, Avenida Brasil, 4365, Manguinhos Pavilhão Leônidas Deane - sl. 209, CEP: 21040-900, Rio de Janeiro, RJ, Brasil;Laboratório de Imunologia Clínica, IOC, Fiocruz, Rio de Janeiro, RJ, Brasil;Laboratório de Imunoparasitologia, IOC, Fiocruz, Rio de Janeiro, RJ, Brasil;Programa de Computação Científica, Presidência, Fiocruz, Rio de Janeiro, RJ, Brasil;

关键词: Major Histocompatibility Complex;    Root Mean Square Deviation;    Visceral Leishmaniasis;    Leishmaniasis;    Major Histocompatibility Complex Molecule;   

DOI  :  10.1186/1471-2172-12-44

 received in 2011-02-14, accepted in 2011-08-08,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLeishmania parasites have been reported to interfere and even subvert their host immune responses to enhance their chances of survival and proliferation. Experimental Leishmania infection in mice has been widely used in the identification of specific parasite virulence factors involved in the interaction with the host immune system. Cysteine-proteinase B (CPB) is an important virulence factor in parasites from the Leishmania (Leishmania) mexicana complex: it inhibits lymphocytes Th1 and/or promotes Th2 responses either through proteolytic activity or through epitopes derived from its COOH-terminal extension. In the present study we analyzed the effects of Leishmania (Leishmania) amazonensis CPB COOH-terminal extension-derived peptides on cell cultures from murine strains with distinct levels of susceptibility to infection: BALB/c, highly susceptible, and CBA, mildly resistant.ResultsPredicted epitopes, obtained by in silico mapping, displayed the ability to induce cell proliferation and expression of cytokines related to Th1 and Th2 responses. Furthermore, we applied in silico simulations to investigate how the MHC/epitopes interactions could be related to the immunomodulatory effects on cytokines, finding evidence that specific interaction patterns can be related to in vitro activities.ConclusionsBased on our results, we consider that some peptides from the CPB COOH-terminal extension may influence host immune responses in the murine infection, thus helping Leishmania survival.

【 授权许可】

Unknown   
© Pereira et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.