Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum

BMC Genomics

Research Article

Agnès Méreau¹  Karl Gordon²  Thomas Walsh³  Owain Edwards³  Dominique Lavenier⁴  Nathalie Leterme⁵  Stéphanie Jaubert-Possamai⁵  Denis Tagu⁵  Fabrice Legeai⁶  Jacques Nicolas⁷  Guillaume Rizk⁸ 

[1] CNRS, Univ Rennes 1, UMR 6061, IFR 140, Rennes, France;CSIRO Entomology, Centre for Environment and Life Sciences (CELS), 6014, Floreat Park, WA, Australia;CSIRO Entomology, GPO Box 1700, ACT 2601, Canberra, Australia;ENS Cachan/IRISA Campus de Beaulieu, 35042, Rennes, France;INRA, UMR1099 BiO3P, Domaine de la Motte, F-35653, Le Rheu, France;INRA, UMR1099 BiO3P, Domaine de la Motte, F-35653, Le Rheu, France;INRIA Centre Rennes - Bretagne Atlantique, GenOuest, Campus de Beaulieu, 35042, Rennes, France;INRIA Centre Rennes - Bretagne Atlantique, GenOuest, Campus de Beaulieu, 35042, Rennes, France;Université de Rennes I/IRISA Campus de Beaulieu, 35042, Rennes, France;

关键词: Deep Sequencing;    Microfluidic Chip;    Mature miRNAs;    miRNA Precursor;    Parthenogenetic Female;   

DOI  :  10.1186/1471-2164-11-281

 received in 2009-07-07, accepted in 2010-05-05,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPost-transcriptional regulation in eukaryotes can be operated through microRNA (miRNAs) mediated gene silencing. MiRNAs are small (18-25 nucleotides) non-coding RNAs that play crucial role in regulation of gene expression in eukaryotes. In insects, miRNAs have been shown to be involved in multiple mechanisms such as embryonic development, tissue differentiation, metamorphosis or circadian rhythm. Insect miRNAs have been identified in different species belonging to five orders: Coleoptera, Diptera, Hymenoptera, Lepidoptera and Orthoptera.ResultsWe developed high throughput Solexa sequencing and bioinformatic analyses of the genome of the pea aphid Acyrthosiphon pisum in order to identify the first miRNAs from a hemipteran insect. By combining these methods we identified 149 miRNAs including 55 conserved and 94 new miRNAs. Moreover, we investigated the regulation of these miRNAs in different alternative morphs of the pea aphid by analysing the expression of miRNAs across the switch of reproduction mode. Pea aphid microRNA sequences have been posted to miRBase: http://microrna.sanger.ac.uk/sequences/ConclusionsOur study has identified candidates as putative regulators involved in reproductive polyphenism in aphids and opens new avenues for further functional analyses.

【 授权许可】

Unknown   
© Legeai et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.