Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

BMC Cell Biology

Research Article

Li Huang¹  Yuyu Li¹  Shujuan Zou¹  Zhiai Hu¹  Chenchen Zhou¹  Xin Wang¹  Yang Xu¹ 

[1] Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section, Renmin South Road, 610041, Chengdu, China;State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section, Renmin South Road, 610041, Chengdu, China;

关键词: OCCM-30 cell;    Intermittent parathyroid hormone (PTH);    Mechanical strain;    Cementogenesis;    Tooth root resorption;   

DOI  :  10.1186/s12860-017-0133-0

 received in 2016-11-29, accepted in 2017-04-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundExternal root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment.ResultsHigher levels of cementogenesis- and differentiation-related biomarkers (bone sialoprotein (BSP), osteocalcin (OCN), Collagen type I (COL1) and Osterix (Osx)) were shown in 1–3 cycles of intermittent PTH treated groups than the control group. Additionally, intermittent PTH increased alkaline phosphatase (ALP) activity and mineralized nodules formation, as measured by ALP staining, quantitative ALP assay, Alizarin red S staining and quantitative calcium assay. The morphology of OCCM-30 cells changed after mechanical strain exertion. Expression of BSP, ALP, OCN, osteopontin (OPN) and Osx was restrained after 18 h mechanical strain. Furthermore, intermittent PTH significantly increased the expression of cementogenesis- and differentiation-related biomarkers in mechanical strain treated OCCM-30 cells.ConclusionsTaken together, these data suggested that intermittent PTH promoted cementum formation through activating cementogenesis- and differentiation-related biomarkers, and attenuated the catabolic effects of mechanical strain in immortalized cementoblasts OCCM-30.

【 授权许可】

CC BY   
© The Author(s). 2017