Comparison of T2 and T2*-weighted MR molecular imaging of a mouse model of glioma

BMC Medical Imaging

Research Article

Randy Tyson¹  Abedelnasser Abulrob²  Garnette R Sutherland³  Tadeusz Foniok³  David Rushforth³  Barbara Blasiak⁴  Boguslaw Tomanek⁵  Andre Obenaus⁶  Samuel Barnes⁶  John Matyas⁷  Dragana Ponjevic⁷  Umar Iqbal⁸  Wladyslaw P Weglarz⁹ 

[1] Alberta Innovates – Technology Futures, 3608 33 Street NW, T2L 2A6, Calgary, Alberta, Canada;Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, Ontario, Canada;Human Health Therapeutics Portfolio, National Research Council of Canada, K1A 0R6, Ottawa, Ontario, Canada;Department of Clinical Neurosciences and Radiology, University of Calgary, 3330 Hospital Dr NW, T2N 4N1, Calgary, Alberta, Canada;Department of Clinical Neurosciences and Radiology, University of Calgary, 3330 Hospital Dr NW, T2N 4N1, Calgary, Alberta, Canada;Polish Academy of Sciences, Institute of Nuclear Physics, Krakow,152 Radzikowskiego, 31-342, Krakow, Malopolska, Poland;Department of Clinical Neurosciences and Radiology, University of Calgary, 3330 Hospital Dr NW, T2N 4N1, Calgary, Alberta, Canada;Polish Academy of Sciences, Institute of Nuclear Physics, Krakow,152 Radzikowskiego, 31-342, Krakow, Malopolska, Poland;Thunder Bay Regional Research Institute, 980 Oliver Road, P7B 6V4, Thunder Bay, Ontario, Canada;Departments of Radiation Medicine, Radiology, Pediatrics, Loma Linda University Chan Shun Pavilion, Room A101011175 Campus Street, 92354, Loma Linda, California, USA;Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Dr NW, T2N 4N1, Calgary, Alberta, Canada;Human Health Therapeutics Portfolio, National Research Council of Canada, K1A 0R6, Ottawa, Ontario, Canada;Polish Academy of Sciences, Institute of Nuclear Physics, Krakow,152 Radzikowskiego, 31-342, Krakow, Malopolska, Poland;

关键词: Contrast-to-noise ratio;    MRI;    Molecular MRI;    Contrast agents;    Glioma;   

DOI  :  10.1186/1471-2342-13-20

 received in 2013-02-18, accepted in 2013-07-17,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundStandard MRI has been used for high-grade gliomas detection, albeit with limited success as it does not provide sufficient specificity and sensitivity to detect complex tumor structure. Therefore targeted contrast agents based on iron oxide, that shorten mostly T2 relaxation time, have been recently applied. However pulse sequences for molecular imaging in animal models of gliomas have not been yet fully studied. The aim of this study was therefore to compare contrast-to-noise ratio (CNR) and explain its origin using spin-echo (SE), gradient echo (GE), GE with flow compensation (GEFC) as well as susceptibility weighted imaging (SWI) in T2 and T2* contrast-enhanced molecular MRI of glioma.MethodsA mouse model was used. U87MGdEGFRvIII cells (U87MG), derived from a human tumor, were injected intracerebrally. A 9.4 T MRI system was used and MR imaging was performed on the 10 day after the inoculation of the tumor. The CNR was measured prior, 20 min, 2 hrs and 24 hrs post intravenous tail administration of glioma targeted paramagnetic nanoparticles (NPs) using SE, SWI, GE and GEFC pulse sequences.ResultsThe results showed significant differences in CNR among all pulse sequences prior injection. GEFC provided higher CNR post contrast agent injection when compared to GE and SE. Post injection CNR was the highest with SWI and significantly different from any other pulse sequence.ConclusionsMolecular MR imaging using targeted contrast agents can enhance the detection of glioma cells at 9.4 T if the optimal pulse sequence is used. Hence, the use of flow compensated pulse sequences, beside SWI, should to be considered in the molecular imaging studies.

【 授权许可】

Unknown   
© Blasiak et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.