Anti-arthritic activity of ethanol extract of Claoxylon indicum on Freund’s complete adjuvant-induced arthritis in mice

BMC Complementary and Alternative Medicine

Research Article

Qi-wen Wang¹  Jian-wei Chen¹  Jian Zuo¹  Yong Chen²  Xiang Li² 

[1] College of Pharmacy, Nanjing University of Chinese Medicine, POB 210023, Nanjing, China;College of Pharmacy, Nanjing University of Chinese Medicine, POB 210023, Nanjing, China;Jiangsu Key Laboratory of Therapeutic Material of Chinese Medicine, POB 210023, Nanjing, China;

关键词: Claoxylon indicum;    Rheumatoid arthritis (RA);    Adjuvant arthritis;    Inflammation;   

DOI  :  10.1186/s12906-016-1500-7

 received in 2016-07-08, accepted in 2016-12-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundClaoxylon indicum Hassk. (Euphorbiaceae), named Diu Le Bang, have functions of dehumidification and relieving swelling pain, and is used as a folk medicine to treat Rheumatoid arthritis (RA), lumbocrural pain and foot edema in the south of China. The aim of the present study was to investigate the anti-arthritic activity of the ethanol extract of Claoxylon indicum (CIE) on mice with adjuvant induced joint arthritis.MethodsAdjuvant arthritis was induced in mice by subcutaneous injection of complete Freund’s adjuvant into the plantar surface of right hind paw. Arthritis severity was evaluated by arthritic score, hind paws oedema and spleen index, and histological examinations. Serum samples were collected for determination of malondialdehyde (MDA) and alkaline phosphatase (ALP) levels. The expression of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in the specimens of knee joints was determined by standard immunohistochemical techniques.ResultsCIE administration (0.4 and 0.8 g/kg) suppressed the inflammatory responses in the joints of adjuvant-induced arthritis (AIA) mice, suggested by the modulatory effects on paw swelling, hyperplasia of lymphatic tissues and synovial membrane. It also decreased the levels of MDA and ALP in serum and downregulated the expression of IL-1β and TNF-α in the arthritic joints of AIA mice.ConclusionThese results suggested that CIE possessed substantial anti-arthritic activity due to immumodepression and regulation of cytokines. CIE may be a potential candidate for the treatment of RA.

【 授权许可】

CC BY   
© The Author(s). 2017