Bone turnover in passive smoking female rat: relationships to change in bone mineral density

BMC Musculoskeletal Disorders

Research Article

Wei Luo¹  Wen-shuo Xu¹  Shu-guang Gao¹  Jian Tian¹  Wei Jiang¹  Mai Xu¹  Guang-hua Lei²  Kang-hua Li²  Hong Shen³ 

[1] Department of Orthopaedics, Xiangya Hospital, Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China;Department of Orthopaedics, Xiangya Hospital, Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China;Orthopaedics Institute of Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China;Medical Research Center, Xiangya Hospital, Central South University, No.87 Xiangya Road, 410008, Changsha, Hunan, China;

关键词: Bone Mineral Density;    Nicotine;    Bone Turnover;    Smoke Exposure;    Lumbar Vertebra;   

DOI  :  10.1186/1471-2474-12-131

 received in 2010-12-26, accepted in 2011-06-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMany studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoking on bone mineral density (BMD) and bone turnover and the relationship between BMD and bone turnover in female rat.MethodsForty-eight female Wistar rats were randomized into six groups: 2-month, 3-month,4-month smoke-exposed rats and their controls. A rat model of passive cigarette smoking was prepared by breeding female rats in a cigarette-smoking box for 2, 3 or 4 months. Serums were analyzed for levels of osteocalcin, bone-specific alkaline phosphatase (b-ALP) and Tartrate-resistant acid phosphatase 5b (TRACP 5b). BMD was assessed at lumbar vertebrae and femur by dual energy X-ray absorptiometry in passive smoking rats and in control rats.ResultsBMD of lumbar spine and femur was lower in 4-month smoke-exposed female rats than that in controls. However, there was no significant difference in serum osteocalcin levels between smoke-exposed rats and controls. Significantly lower b-ALP and higher TRACP 5b were found in the 3-month or 4-month smoke-exposed rats compared to controls. Subsequent analysis showed that b-ALP positively correlated with BMD of the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed female rats. Furthermore, TRACP 5b levels negatively correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed female rats.ConclusionOur data suggest that smoke exposure can inhibit bone formation and increase bone resorption. The hazardous effects of passive smoking on bone status are associated with increased bone turnover in female rat.

【 授权许可】

Unknown   
© Gao et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.