Characterization of the ovine ribosomal protein SA gene and its pseudogenes

BMC Genomics

Research Article

Ane Marcos-Carcavilla¹  Mario Van Poucke²  Alex Van Zeveren²  Alice Van den Broeke²  Luc J Peelman²  Hélène Hayes³  Maud Bertaud³  Karine Hugot³ 

[1] Departamento de Mejora Genética Animal, INIA, Ctra La Coruña Km 7.5, 28040, Madrid, Spain;Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820, Merelbeke, Belgium;INRA, UMR 1313 Génétique Animale et Biologie Intégrative, F78350, Jouy-en-Josas, France;

关键词: Bacterial Artificial Chromosome;    EGCG;    Bacterial Artificial Chromosome Clone;    Bovine Genome;    Multicopy Gene Family;   

DOI  :  10.1186/1471-2164-11-179

 received in 2009-12-09, accepted in 2010-03-16,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe ribosomal protein SA (RPSA), previously named 37-kDa laminin receptor precursor/67-kDa laminin receptor (LRP/LR) is a multifunctional protein that plays a role in a number of pathological processes, such as cancer and prion diseases. In all investigated species, RPSA is a member of a multicopy gene family consisting of one full length functional gene and several pseudogenes. Therefore, for studies on RPSA related pathways/pathologies, it is important to characterize the whole family and to address the possible function of the other RPSA family members. The present work aims at deciphering the RPSA family in sheep.ResultsIn addition to the full length functional ovine RPSA gene, 11 other members of this multicopy gene family, all processed pseudogenes, were identified. Comparison between the RPSA transcript and these pseudogenes shows a large variety in sequence identities ranging from 99% to 74%. Only one of the 11 pseudogenes, i.e. RPSAP7, shares the same open reading frame (ORF) of 295 amino acids with the RPSA gene, differing in only one amino acid. All members of the RPSA family were annotated by comparative mapping and fluorescence in situ hybridization (FISH) localization. Transcription was investigated in the cerebrum, cerebellum, spleen, muscle, lymph node, duodenum and blood, and transcripts were detected for 6 of the 11 pseudogenes in some of these tissues.ConclusionsIn the present work we have characterized the ovine RPSA family. Our results have revealed the existence of 11 ovine RPSA pseudogenes and provide new data on their structure and sequence. Such information will facilitate molecular studies of the functional RPSA gene taking into account the existence of these pseudogenes in the design of experiments. It remains to be investigated if the transcribed members are functional as regulatory non-coding RNA or as functional proteins.

【 授权许可】

Unknown   
© Van den Broeke et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.