Co-synergism of endophyte Penicillium resedanum LK6 with salicylic acid helped Capsicum annuumin biomass recovery and osmotic stress mitigation

BMC Microbiology

Research Article

Ahmed Al-Harrasi¹  Ahmed Al-Rawahi¹  Abdul Latif Khan²  Muhammad Hamayun³  Muhammad Waqas⁴  In-Jung Lee⁴ 

[1] Department of Biological Sciences and Chemistry, University of Nizwa, Nizwa, Oman;Department of Biological Sciences and Chemistry, University of Nizwa, Nizwa, Oman;School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea;Kohat University of Science & Technology, Kohat, Pakistan;Department of Botany, Abdul Wali Khan University, Mardan, Pakistan;School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea;

关键词: Penicillium resedanum LK6;    Osmotic stress;    Salicylic acid;    Antioxidants;    Biomass recovery;    Capsicum annuum;   

DOI  :  10.1186/1471-2180-13-51

 received in 2012-12-09, accepted in 2013-02-20,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundWater-deficiency adversely affects crop growth by generating reactive oxygen species (ROS) at cellular level. To mitigate such stressful events, it was aimed to investigate the co-synergism of exogenous salicylic acid (SA) and symbiosis of endophytic fungus with Capsicum annuum L. (pepper).ResultsThe findings of the study showed that exogenous SA (10-6 M) application to endophyte (Penicillium resedanum LK6) infected plants not only increased the shoot length and chlorophyll content but also improved the biomass recovery of pepper plants under polyethylene glycol (15%) induced osmotic stress (2, 4 and 8 days). Endophyte-infected plants had low cellular injury and high photosynthesis rate. SA also enhanced the colonization rate of endophyte in the host-plant roots. Endophyte and SA, in combination, reduced the production of ROS by increasing the total polyphenol, reduce glutathione, catalase, peroxidase and polyphenol oxidase as compared to control plants. Osmotic stress pronounced the lipid peroxidation and superoxide anions formation in control plants as compared to endophyte and SA-treated plants. The endogenous SA contents were significantly higher in pepper plants treated with endophyte and SA under osmotic stress as compared to control.ConclusionEndophytic fungal symbiosis and exogenous SA application can help the plants to relieve the adverse effects of osmotic stress by decreasing losses in biomass as compared to non-inoculated plants. These findings suggest that SA application positively impact microbial colonization while in combination, it reprograms the plant growth under various intervals of drought stress. Such symbiotic strategy can be useful for expanding agriculture production in drought prone lands.

【 授权许可】

Unknown   
© Khan et al; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.