Application of concentrated deep sea water inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice

BMC Complementary and Alternative Medicine

Research Article

Chang-Ju Kim¹  Jeonghyun Son²  Jong-Phil Bak²  Ee-Hwa Kim²  Yong-Min Kim³ 

[1] Department of Physiology, College of Medicine, Kyung Hee University, 130-701, Seoul, South Korea;The Clinical Trial Center for Bio-Industry, Semyung University, 309-711, Jecheon, South Korea;The Clinical Trial Center for Bio-Industry, Semyung University, 309-711, Jecheon, South Korea;Department of Physiology, College of Medicine, Kyung Hee University, 130-701, Seoul, South Korea;

关键词: Atopic Dermatitis;    Pimecrolimus;    Dorsal Skin;    Spleen Lymphocyte;    Epidermal Thickness;   

DOI  :  10.1186/1472-6882-12-108

 received in 2012-01-06, accepted in 2012-07-05,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMineral water from deep-sea bedrock, formed over thousands of years, is rich in minerals such as Ca, Mg, Na, K, Fe and others. Our present study was to investigate the preventive effects of natural deep-sea water on developing atopic dermatitis (AD).MethodsWe elicited AD by application of DNCB (2,4-dinitro-chlorobezene) in Nc/Nga mouse dorsal skin. Deep Sea water (DSW) was filtered and concentrated by a nanofiltration process and reverse osmosis. We applied concentrated DSW (CDSW) to lesions five times per week for six weeks, followed by evaluation. 1% pimecrolimus ointment was used as positive control. The severity of skin lesions was assessed macroscopically and histologically. Levels of inflammatory mediators and cytokines in the serum were detected by Enzyme-linked immunosorbent assay (ELISA) and the levels of CD4+ and CD8+ spleen lymphocytes were determined by flow cytometry analysis.ResultsDNCB-treated mice showed atopic dermatitis-like skin lesions. Treatment of mice with CDSW reduced the severity of symptoms in the skin lesions, including edema, erythema, dryness, itching, and transepidermal water loss (TEWL). Histological analyses demonstrated that epidermal thickness and infiltration of inflammatory cells were decreased after CDSW treatment. Given these interesting observations, we further evaluated the effect of CDSW on immune responses in this AD model. Treatment AD mice with CDSW inhibited up-regulation of IgE, histamine, and pro-inflammatory cytokines in the serum. Also, the CD4+/CD8+ ratio in spleen lymphocyte was down-regulated after treatment with CDSW. Finally, cytokines, especially IL-4 and IL-10 which are important for Th2 cell development, were reduced.ConclusionsOur data suggests that topical application of CDSW could be useful in preventing the development of atopic dermatitis.

【 授权许可】

Unknown   
© Bak et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.