NOX2-generated oxidative stress is associated with severity of ultrasound liver steatosis in patients with non-alcoholic fatty liver disease

BMC Gastroenterology

Research Article

Cristina Nocella¹  Pasquale Pignatelli¹  Simona Bartimoccia¹  Licia Polimeni¹  Francesco Violi¹  Maria Del Ben¹  Roberto Carnevale¹  Lorenzo Loffredo¹  Francesco Baratta¹  Francesco Angelico² 

[1] Department of Internal Medicine and Medical Specialties, Sapienza University, Rome, Italy;Department of Public Health and Infectious Disease, Sapienza University, Rome, Italy;I Clinica Medica – Policlinico Umberto I, Viale del Policlinico 155, 00161, Rome, Italy;

关键词: Oxidative stress;    Non-alcoholic fatty liver;    8-iso-PGF2α;    sNOX2-dp;    Metabolic syndrome;   

DOI  :  10.1186/1471-230X-14-81

 received in 2013-08-08, accepted in 2014-04-16,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundChronic oxidative stress is one of the key mechanisms responsible for disease progression in non-alcoholic fatty liver disease. However, so far, few studies reported increased circulating levels of oxidative stress markers in patients with non-alcoholic fatty liver and no study has been performed with newer markers of systemic oxidative stress. The aim was to assess the relationship between urinary 8-iso-prostaglandin F2α and serum soluble NOX2-derived peptide and the severity of liver steatosis in subjects with non-alcoholic fatty liver.MethodsThe study was performed in 264 consecutive patients referred for suspected metabolic disease. Steatosis was defined according to Hamaguchi ultrasonographic criteria. Oxidative stress was assessed by urinary 8-iso- prostaglandin F2α and serum soluble NOX2-derived peptide levels.ResultsPatients with non-alcoholic fatty liver had higher (p < 0.001) mean values of urinary 8-iso-PGF2α and of serum soluble NOX2-derived peptide, alanine aminotransferase, Cytokeratin-18 and homeostasis model of insulin resistance and lower values of serum adiponectin as compared to those without. Prevalence of metabolic syndrome and of its clinical features was significantly higher in patients with non-alcoholic fatty liver. Same findings were also observed after the exclusion of obese subjects, or subjects with diabetes or with metabolic syndrome and in those not taking statin medication. In addition, the levels of urinary 8-iso-PGF2α were independent predictors of non-alcoholic fatty liver and a strong association of urinary 8-iso-PGF2α and of serum soluble NOX2-derived peptide with the severity of steatosis at ultrasound was also observed.ConclusionsWe demonstrated increased markers of oxidative stress in subjects with non-alcoholic fatty liver. Urinary 8-iso-PGF2α and serum soluble NOX2-derived peptide levels were independent from obesity, diabetes and metabolic syndrome and increased with the severity of liver steatosis at ultrasound.

【 授权许可】

Unknown   
© Del Ben et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.