Functional and evolutionary analyses of the miR156 and miR529 families in land plants

BMC Plant Biology

Research Article

Michel Vincentz¹  Guilherme Targino Valente²  Eder Marques da Silva³  Fabio Tebaldi Silveira Nogueira³  Geraldo Felipe Ferreira e Silva³  Carlos Hernan Barrera Rojas⁴  Edna Gicela Ortiz Morea⁴ 

[1] Centro de Biologia Molecular e Engenharia Genetica (CBMEG), Universidade Estadual de Campinas, Campinas, Sao Paulo, Brazil;Departmento de Bioprocessos e Biotecnologia, Universidade Estadual Paulista (UNESP), Botucatu, Sao Paulo, Brazil;Laboratório de Genetica Molecular do Desenvolvimento Vegetal, Departamento de Ciencias Biológicas, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de Sao Paulo, Avenida Padua Dias, 11, 13418-900, Piracicaba, Sao Paulo, Brazil;Laboratório de Genetica Molecular do Desenvolvimento Vegetal, Departamento de Ciencias Biológicas, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de Sao Paulo, Avenida Padua Dias, 11, 13418-900, Piracicaba, Sao Paulo, Brazil;Departamento de Genética, Instituto de Biociencias, Universidade Estadual Paulista (UNESP), Botucatu, Sao Paulo, Brazil;

关键词: miR156;    miR529;    Evolution;    Arabidopsis;    Eudicots;   

DOI  :  10.1186/s12870-016-0716-5

 received in 2015-09-17, accepted in 2016-01-18,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are important regulatory elements of gene expression. Similarly to coding genes, miRNA genes follow a birth and death pattern of evolution likely reflecting functional relevance and divergence. For instance, miRNA529 is evolutionarily related to miRNA156 (a highly conserved miRNA in land plants), but it is lost in Arabidopsis thaliana. Interestingly, both miRNAs target sequences overlap in some members of the SQUAMOSA promoter-binding protein like (SPL) family, raising important questions regarding the diversification of the miR156/miR529-associated regulatory network in land plants.ResultsIn this study, through phylogenic reconstruction of miR156/529 target sequences from several taxonomic groups, we have found that specific eudicot SPLs, despite miRNA529 loss, retained the corresponding target site. Detailed molecular evolutionary analyses of miR156/miR529-target sequence showed that loss of miR529 in core eudicots, such as Arabidopsis, is correlated with a more relaxed selection of the miRNA529 specific target element, while miRNA156-specific target sequence is under stronger selection, indicating that these two target sites might be under distinct evolutionary constraints. Importantly, over-expression in Arabidopsis of MIR529 precursor from a monocot, but not from a basal eudicot, demonstrates specific miR529 regulation of AtSPL9 and AtSPL15 genes, which contain conserved responsive elements for both miR156 and miR529.ConclusionsOur results suggest loss of functionality of MIR529 genes in the evolutionary history of eudicots and show that the miR529-responsive element present in some eudicot SPLs is still functional. Our data support the notion that particular miRNA156 family members might have compensated for the loss of miR529 regulation in eudicot species, which concomitantly may have favored diversification of eudicot SPLs.

【 授权许可】

CC BY   
© Morea et al. 2016