Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing

BMC Genomics

Research Article

Xumin Wang¹  Shichao Liu²  Zhonghua Liu²  Jun Wu³  Yangli Pei⁴  Qiuyan Li⁴  Ning Li⁴  Jianyong Han⁴  Wei Zhang⁴  Suying Cao⁵  Bing Lim⁶  Xiaoan Ruan⁶ 

[1] Beijing Institute of Genomics, Chinese academy of Sciences, Beijing, China;College of Life Science, Northeast Agricultural University, Haerbin, China;Novogene Bioinformatics Institute, Beijing, China;State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China;State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China;Animal Science and Technology College, Beijing University of Agriculture, Beijing, China;Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore, Singapore;

关键词: Fatty Acid Metabolism;    Inner Cell Mass;    Oct4 Expression;    Zygotic Genome Activation;    Reconstructed Embryo;   

DOI  :  10.1186/1471-2164-15-4

 received in 2013-05-12, accepted in 2013-12-26,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundBecause few studies exist to describe the unique molecular network regulation behind pig pre-implantation embryonic development (PED), genetic engineering in the pig embryo is limited. Also, this lack of research has hindered derivation and application of porcine embryonic stem cells and porcine induced pluripotent stem cells (iPSCs).ResultsWe identified and analyzed the genome wide transcriptomes of pig in vivo-derived and somatic cell nuclear transferred (SCNT) as well as mouse in vivo-derived pre-implantation embryos at different stages using mRNA deep sequencing. Comparison of the pig embryonic transcriptomes with those of mouse and human pre-implantation embryos revealed unique gene expression patterns during pig PED. Pig zygotic genome activation was confirmed to occur at the 4-cell stage via genome-wide gene expression analysis. This activation was delayed to the 8-cell stage in SCNT embryos. Specific gene expression analysis of the putative inner cell mass (ICM) and the trophectoderm (TE) revealed that pig and mouse pre-implantation embryos share regulatory networks during the first lineage segregation and primitive endoderm differentiation, but not during ectoderm commitment. Also, fatty acid metabolism appears to be a unique characteristic of pig pre-implantation embryonic development. In addition, the global gene expression patterns in the pig SCNT embryos were different from those in in vivo-derived pig embryos.ConclusionsOur results provide a resource for pluripotent stem cell engineering and for understanding pig development.

【 授权许可】

Unknown   
© Cao et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.