A comparison of 12-gene colon cancer assay gene expression in African American and Caucasian patients with stage II colon cancer

BMC Cancer

Research Article

Awais Javed¹  Ahmed Y. Javed¹  Raymond U. Osarogiagbon¹  Calvin Y. Chao²  Ruixiao Lu²  Trafina Jadhav³  Upender Manne³  James Posey³  Fade A. Mahmoud⁴  Rangaswamy Govindarajan⁴ 

[1] Boston Baskin Cancer Foundation, Baptist Cancer Center, Memphis, TN, USA;Genomic Health Inc., Redwood City, CA; Genomic Health, Inc., Redwood City, CA, USA;University of Alabama at Birmingham Comprehensive Cancer Center, Birmingham, AL, USA;University of Arkansas for Medical Sciences, 4301 W Markham, Slot 508, 72205, Little Rock, AR, USA;

关键词: Colon cancer;    12-gene assay;    Gene expression;    Stage II;    African-American;    Caucasian;   

DOI  :  10.1186/s12885-016-2365-3

 received in 2015-12-17, accepted in 2016-05-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAfrican American (AA) colon cancer patients have a worse prognosis than Caucasian (CA) colon cancer patients, however, reasons for this disparity are not well understood. To determine if tumor biology might contribute to differential prognosis, we measured recurrence risk and gene expression using the Oncotype DX® Colon Cancer Assay (12-gene assay) and compared the Recurrence Score results and gene expression profiles between AA patients and CA patients with stage II colon cancer.MethodsWe retrieved demographic, clinical, and archived tumor tissues from stage II colon cancer patients at four institutions. The 12-gene assay and mismatch repair (MMR) status were performed by Genomic Health (Redwood City, California). Student’s t-test and the Wilcoxon rank sum test were used to compare Recurrence Score data and gene expression data from AA and CA patients (SAS Enterprise Guide 5.1).ResultsSamples from 122 AA and 122 CA patients were analyzed. There were 118 women (63 AA, 55 CA) and 126 men (59 AA, 67 CA). Median age was 66 years for AA patients and 68 for CA patients. Age, gender, year of surgery, pathologic T-stage, tumor location, the number of lymph nodes examined, lymphovascular invasion, and MMR status were not significantly different between groups (p = 0.93). The mean Recurrence Score result for AA patients (27.9 ± 12.8) and CA patients (28.1 ± 11.8) was not significantly different and the proportions of patients with high Recurrence Score values (≥41) were similar between the groups (17/122 AA; 15/122 CA). None of the gene expression variables, either single genes or gene groups (cell cycle group, stromal group, BGN1, FAP, INHBA1, Ki67, MYBL2, cMYC and GADD45B), was significantly different between the racial groups. After controlling for clinical and pathologic covariates, the means and distributions of Recurrence Score results and gene expression profiles showed no statistically significant difference between patient groups.ConclusionThe distribution of Recurrence Score results and gene expression data was similar in a cohort of AA and CA patients with stage II colon cancer and similar clinical characteristics, suggesting that tumor biology, as represented by the 12-gene assay, did not differ between patient groups.

【 授权许可】

CC BY   
© Govindarajan et al. 2016