The influence of astragalus polysaccharide and β-elemene on LX-2 cell growth, apoptosis and activation

BMC Gastroenterology

Research Article

Cheng-hua Li¹  Ming-hui Yang²  Shao-dan Li²  Yong-qi Dou²  Yi Liu²  Jin Zheng³  Qin-you Ren⁴  Heng-jun Shi⁴  Lu Li⁴  Li-tian Ma⁴  Yi Zhang⁴  Hui Zhang⁴ 

[1] Department of Oncology, Shaanxi province Hospital of Traditional Chinese Medicine, 710003, Xi’an, China;Department of Traditional Chinese Medicine, General Hospital of People’s Liberation Army, 100853, Beijing, China;Department of Traditional Chinese Medicine, General Hospital of People’s Liberation Army, 100853, Beijing, China;Department of Traditional Chinese Medicine, Tangdu Hospital, The Fourth Military Medical University, 710038, Xi’an, China;Department of Traditional Chinese Medicine, Tangdu Hospital, The Fourth Military Medical University, 710038, Xi’an, China;

关键词: Astragalus polysaccharide;    β-elemene;    Hepatic stellate cells;   

DOI  :  10.1186/s12876-014-0224-8

 received in 2014-06-24, accepted in 2014-12-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundActivated hepatic stellate cells are the main source of excessive collagen deposition in liver fibrosis. Here we report the inhibitory effects of the combinational treatment of two natural products, astragalus polysaccharide (APS) and β-elemene (ELE) on the activation of human liver hepatic stellate cell line LX-2 cells.MethodsCultured LX-2 cells were treated with different concentrations of APS or ELE for 24 or 48 hours. Cell viability/apoptosis was measured by MTT assay and Annexin V/PI staining , activation related genes including α-SMA and CD44 expressions were measured by real-time PCR and western blot respectively.ResultsThe majority of LX-2 cells showed morphological change in the presence of APS or ELE for 24 hours. Treatment with APS + ELE for 24 or 48 hours significantly inhabited the cell proliferation compared with APS or ELE treatment alone on LX-2 cells. APS + ELE may block the up-regulation of α-SMA and CD44 both in mRNA and protein levels through TGF-β pathway in LX-2 cells.ConclusionAPS or ELE treatment alone on LX-2 cells could inhibit cell proliferation and induce apoptosis. The combinational treatment using APS + ELE significantly increased the killing efficiency on LX-2 cells. α-SMA and CD44 expressions was inhibited upon APS + ELE treatment through TGF-β pathway in LX-2 cells. The results indicated a novel treatment using natural products for liver diseases with anti-fibrotic effect.

【 授权许可】

Unknown   
© Zheng et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.