Differential expression of conserved and novel microRNAs during tail regeneration in the lizard Anolis carolinensis

BMC Genomics

Research Article

Walter L. Eckalbar¹  Elizabeth D. Hutchins²  Kenro Kusumi²  Marco Mangone³  Justin M. Wolter³ 

[1] School of Life Sciences, Arizona State University, 85287, Tempe, AZ, USA;Department of Bioengineering and Therapeutic Sciences, Institute for Human Genetics, University of California San Francisco, 94143, San Francisco, CA, USA;School of Life Sciences, Arizona State University, 85287, Tempe, AZ, USA;Neurogenomics Division, Translational Genomics Research Institute, 85004, Phoenix, AZ, USA;School of Life Sciences, Arizona State University, 85287, Tempe, AZ, USA;Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, 85287, Tempe, AZ, USA;

关键词: microRNA;    Regeneration;    Transcriptome;    Lizard;    Reptile;    Gene expression;    Tail;    Muscle;    Brain;   

DOI  :  10.1186/s12864-016-2640-3

 received in 2015-12-31, accepted in 2016-04-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLizards are evolutionarily the most closely related vertebrates to humans that can lose and regrow an entire appendage. Regeneration in lizards involves differential expression of hundreds of genes that regulate wound healing, musculoskeletal development, hormonal response, and embryonic morphogenesis. While microRNAs are able to regulate large groups of genes, their role in lizard regeneration has not been investigated.ResultsMicroRNA sequencing of green anole lizard (Anolis carolinensis) regenerating tail and associated tissues revealed 350 putative novel and 196 known microRNA precursors. Eleven microRNAs were differentially expressed between the regenerating tail tip and base during maximum outgrowth (25 days post autotomy), including miR-133a, miR-133b, and miR-206, which have been reported to regulate regeneration and stem cell proliferation in other model systems. Three putative novel differentially expressed microRNAs were identified in the regenerating tail tip.ConclusionsDifferentially expressed microRNAs were identified in the regenerating lizard tail, including known regulators of stem cell proliferation. The identification of 3 putative novel microRNAs suggests that regulatory networks, either conserved in vertebrates and previously uncharacterized or specific to lizards, are involved in regeneration. These findings suggest that differential regulation of microRNAs may play a role in coordinating the timing and expression of hundreds of genes involved in regeneration.

【 授权许可】

CC BY   
© Hutchins et al. 2016