Blockade of leukocyte haptokinesis and haptotaxis by ketoprofen, diclofenac and SC-560

BMC Immunology

Research Article

Vachtang Kerkadze¹  Eduard Ryschich¹  Jan Schmidt¹  Audrius Parseliunas²  Saulius Paskauskas²  Rainer Nobiling³ 

[1] Department of Surgery, University of Heidelberg, Heidelberg, Germany;Department of Surgery, University of Heidelberg, Heidelberg, Germany;University of Health Sciences, Kaunas, Lithuania;Institute of Physiology, University of Heidelberg, Heidelberg, Germany;

关键词: Ketoprofen;    Polymorphonuclear Leukocyte;    Collagen Matrix;    Leukocyte Recruitment;    Leukocyte Migration;   

DOI  :  10.1186/1471-2172-12-64

 received in 2011-07-28, accepted in 2011-11-12,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundNonsteroidal anti-inflammatory drugs (NSAID) represent a one of the most widely used anti-inflammatory substances. Their anti-inflammatory effects are mainly based on inhibition of cyclooxygenase. The potential direct effect of NSAID on leukocyte migration was poorly investigated. Using time-lapse microscopy and 96-well fluorescence-based assay, we studied the effect of three different NSAID, ketoprofen, diclofenac and SC-560, on leukocyte haptokinesis and haptotaxis in vivo and in vitro.ResultsNSAID induced an immediate inhibiting effect on leukocyte migration both in vitro and in vivo. This effect was dose-dependent and was not restricted to a specific type of leukocytes. The inhibition of leukocyte migration by NSAID was partially re-stored after removal of inhibiting agent. Only complete blockade of leukocyte migration was accompanied by a strong reduction of [Ca2+]i.ConclusionsNSAID strongly supress leukocyte migration. The results of the present study may have important clinical implications since blockade of leukocyte migration can be achieved after topical application of NSAID.

【 授权许可】

Unknown   
© Paskauskas et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.