Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells

BMC Plant Biology

Methodology Article

Franziska Krajinski¹  Silvia Bortfeld¹  Alexander Erban¹  Nicole Gaude¹  Joachim Kopka¹ 

[1] Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476, Potsdam, Germany;

关键词: Arbuscular Mycorrhizal;    Gluconic Acid;    Laser Capture Microdissection;    Primary Metabolite;    Mycorrhizal Root;   

DOI  :  10.1186/s12870-015-0601-7

 received in 2015-02-24, accepted in 2015-09-04,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning.ResultsHere we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells.ConclusionsHigh levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

【 授权许可】

CC BY   
© Gaude et al. 2015